DRP1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-55288

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Fish, Primate

Cited:

Human, Mouse, Rat, Fish

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all DRP1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region within residues 500-600 of the human DRP1 protein. [Swiss-Prot# O00429]

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34622072). Fish reactivity reported in scientific literature (PMID: 25008790).

Localization

Cytoplasm; cytosol. Intracytoplasmic membrane; peripheral mambrane protein. Mainly cytosolic. Also membrane-associated. Localizes to mitochondia at spots of division events. Associated with peroxisomal membranes, it is recruited in part by PEX11B.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DRP1 Antibody - BSA Free

Western Blot: DRP1 AntibodyBSA Free [NB110-55288]

Western Blot: DRP1 AntibodyBSA Free [NB110-55288]

Western Blot: DRP1 Antibody [NB110-55288] - Total protein from human HeLa and MCF7 cells, mouse 3T3 cells and rat PC12 cells was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-DRP1 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: DRP1 Antibody - BSA Free [NB110-55288]

Immunohistochemistry-Paraffin: DRP1 Antibody - BSA Free [NB110-55288]

Immunohistochemistry-Paraffin: DRP1 Antibody [NB110-55288] - Analysis of FFPE tissue section of mouse kidney using DRP1 antibody #NB110-55288 at 1:300. The primary antibody bound to DRP1 protein in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This antibody generated a diffused cytoplasmic staining of DRP1 in the epithelial cells of various tubules and in the cells of glomeruli.
Western Blot: DRP1 AntibodyBSA Free [NB110-55288]

Western Blot: DRP1 AntibodyBSA Free [NB110-55288]

Western Blot: DRP1 Antibody [NB110-55288] - Lane 1: DRP1 knockout. Lane 2: DRP1 wildtype MEFs. Stained with NB110-55288 at 1:500.
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Applications for DRP1 Antibody - BSA Free

Application
Recommended Usage

Flow (Intracellular)

1.0 ug/ml

Flow Cytometry

1:1000

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Immunoprecipitation

reported in scientific literature

Simple Western

1:50

Western Blot

1:500
Application Notes
In Western blot a band is seen at ~81 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 4 reviews rated 5 using NB110-55288 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DRP1

DRP1 (dynamin-related protein 1) is a highly conserved large GTPase protein which implicates in several important structural features of mitochondria, including mitochondrial shape, size, distribution, remodeling and maintenance. It functions in mitochondrial as well as peroxisomal division, and facilitates membrane fission via oligomerization into ring-like structures that wrap around fission site to constrict/split the membranes through a GTP hydrolysis-dependent mechanism. For normal brain development, DRP1 plays an essential role by mediating developmentally-regulated apoptosis during neural tube development. It is also required for a normal rate of cytochrome c release or caspase activation during apoptosis and for mitochondrial fission during mitosis, programmed necrosis execution and also for vesicle transport. Moreover, it is associated with mitochondrial structural and functional alterations such as fragmentation, phosphorylation, SUMOylation, ubiquitination and cell death. Drp1 interacts with amyloid beta, causing abnormal mitochondrial dynamics and neuronal damage in Alzheimer's disease patients, and defective DRP1 is also linked to encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF).

Long Name

Dynamin-1-like protein

Alternate Names

DLP1, DNM1L, DVLP, Dymple, HdynIV

Entrez Gene IDs

10059 (Human); 74006 (Mouse); 114114 (Rat)

Gene Symbol

DNM1L

UniProt

O00429 (Human)

Additional DRP1 Products

Product Documents for DRP1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DRP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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