Dtk/TYRO3 Antibody (JE38-13)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33070
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE38-13
Concentration
1 mg/ml
Product Summary for Dtk/TYRO3 Antibody (JE38-13)
Immunogen
Synthetic peptide within Human Dtk/TYRO3 aa 841-890 / 890. (Uniprot: Q06418)
Localization
Cell membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Dtk/TYRO3 Antibody (JE38-13)
Western Blot: Dtk/TYRO3 Antibody (JE38-13) [NBP3-33070]
Western Blot: Dtk/TYRO3 Antibody (JE38-13) [NBP3-33070] - Western blot analysis of Dtk/TYRO3 on different lysates with Rabbit anti-Dtk/TYRO3 antibody (NBP3-33070) at 1/1,000 dilution.Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: HCT 116 cell lysate (20 ug/Lane)
Lane 3: MCF7 cell lysate (20 ug/Lane)
Lane 4: SH-SY5Y cell lysate (20 ug/Lane)
Lane 5: PC-12 cell lysate (20 ug/Lane)
Lane 6: Human brain tissue lysate (40 ug/Lane)
Predicted band size: 92 kDa
Observed band size: 92 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33070) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for Dtk/TYRO3 Antibody (JE38-13)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Tyro3/Dtk
Long Name
Tyrosine Protein Kinase Receptor TYRO3
Alternate Names
Brt, Dtk, Rse, Sky
Gene Symbol
TYRO3
Additional Tyro3/Dtk Products
Product Documents for Dtk/TYRO3 Antibody (JE38-13)
Product Specific Notices for Dtk/TYRO3 Antibody (JE38-13)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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