Dtk/TYRO3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28635
![Flow (Intracellular): Dtk/TYRO3 Antibody - BSA Free [NBP1-28635]](https://resources.bio-techne.com/images/products/Dtk-TYRO3-Antibody-Flow-Intracellular-NBP1-28635-img0006.jpg "Flow (Intracellular): Dtk/TYRO3 Antibody - BSA Free [NBP1-28635]")
![Western Blot: Dtk/TYRO3 AntibodyBSA Free [NBP1-28635]](https://resources.bio-techne.com/images/products/Dtk-TYRO3-Antibody-Western-Blot-NBP1-28635-img0005.jpg "Western Blot: Dtk/TYRO3 AntibodyBSA Free [NBP1-28635]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Rat (94%). Backed by our 100% Guarantee.
Applications
Validated:
Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.19 mg/ml
Product Specifications
Immunogen
Synthetic peptide made to an internal portion of the human TYRO3 protein (within residues 100-250). [Swiss-Prot# Q06418]
Reactivity Notes
Immunogen sequence has 94% identity to rat.
Localization
Cell membrane; Single-pass type I membrane protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Dtk/TYRO3 Antibody - BSA Free
Western Blot: Dtk/TYRO3 AntibodyBSA Free [NBP1-28635]
Western Blot: Dtk/TYRO3 Antibody [NBP1-28635] - Lane 1: Western blot on normal human brain, Lane 2: Western blot on normal mouse brain.![Immunocytochemistry/ Immunofluorescence: Dtk/TYRO3 Antibody - BSA Free [NBP1-28635]](https://resources.bio-techne.com/images/products/Dtk-TYRO3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-28635-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Dtk/TYRO3 Antibody - BSA Free [NBP1-28635]")
Immunocytochemistry/ Immunofluorescence: Dtk/TYRO3 Antibody - BSA Free [NBP1-28635]
Immunocytochemistry/Immunofluorescence: Dtk/TYRO3 Antibody [NBP1-28635] - Staining of HELA cells using NBP128635 (Green) at a 1:200 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody. Nuclei were visualized using Hoechst 33258 (Blue).![Flow Cytometry: Dtk/TYRO3 Antibody - BSA Free [NBP1-28635]](https://resources.bio-techne.com/images/products/Dtk-TYRO3-Antibody-Flow-Cytometry-NBP1-28635-img0007.jpg "Flow Cytometry: Dtk/TYRO3 Antibody - BSA Free [NBP1-28635]")
Flow Cytometry: Dtk/TYRO3 Antibody - BSA Free [NBP1-28635]
Flow Cytometry: Dtk/TYRO3 Antibody [NBP1-28635] - An intracellular stain was performed on HeLa cells with Dtk/TYRO3 Antibody NBP1-28635AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.Applications for Dtk/TYRO3 Antibody - BSA Free
Application
Recommended Usage
Flow Cytometry
reported in scientific literature (PMID 27034374)
Immunocytochemistry/ Immunofluorescence
1:200
Western Blot
2 ug/ml
Application Notes
In Western blot, a band can be seen at ~96 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 30% Glycerol
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1.19 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Tyro3/Dtk
Long Name
Tyrosine Protein Kinase Receptor TYRO3
Alternate Names
Brt, Dtk, Rse, Sky
Entrez Gene IDs
7301 (Human)
Gene Symbol
TYRO3
UniProt
Additional Tyro3/Dtk Products
Product Documents for Dtk/TYRO3 Antibody - BSA Free
Product Specific Notices for Dtk/TYRO3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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