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Dtk/TYRO3 Antibody [Alexa Fluor® 647]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28635AF647

Novus Biologicals, part of Bio-Techne
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NBP1-28635AF647

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of the human TYRO3 protein (within residues 100-250). [Swiss-Prot# Q06418]

Reactivity Notes

Immunogen sequence has 94% identity to rat.

Localization

Cell membrane; Single-pass type I membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dtk/TYRO3 Antibody [Alexa Fluor® 647]

Flow Cytometry: Dtk/TYRO3 Antibody [Alexa Fluor® 647] [NBP1-28635AF647] - An intracellular stain was performed on HeLa cells with Dtk/TYRO3 Antibody NBP1-28635AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow (Intracellular): Dtk/TYRO3 Antibody [Alexa Fluor 647] [NBP1-28635AF647] - An intracellular stain was performed on U-937 cells with Dtk/TYRO3 Antibody NBP1-28635AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Applications for Dtk/TYRO3 Antibody [Alexa Fluor® 647]

Application
Recommended Usage

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Tyro3/Dtk

Receptor protein tyrosine kinases (RPTKs) are a class of transmembrane signaling molecules. They have been shown to regulate signaling cascades that control synaptic function in the central nervous system (CNS). Tyro3 is one of three RPTKs belonging to the Axl/UFO subfamily, that also includes Axl and Mer. Tyro3 is the most commonly expressed of these receptors in the CNS. It may be involved in the cell adhesion process, and is commonly found in adult tissues with expression mostly in brain, testis, vascular and lymphatic tissue.

Long Name

Tyrosine Protein Kinase Receptor TYRO3

Alternate Names

Brt, Dtk, Rse, Sky

Gene Symbol

TYRO3

Additional Tyro3/Dtk Products

Product Documents for Dtk/TYRO3 Antibody [Alexa Fluor® 647]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Dtk/TYRO3 Antibody [Alexa Fluor® 647]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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