dUTPase Antibody (HL1210) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25445
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1210
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for dUTPase Antibody (HL1210) - Azide and BSA Free
Immunogen
Recombinant protein encompassing a sequence within the center region of human dUTPase. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (86%), Bovine (89%), Chicken (82%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for dUTPase Antibody (HL1210) - Azide and BSA Free
Immunohistochemistry-Paraffin: dUTPase Antibody (HL1210) - Azide and BSA Free [NBP3-25445] -
Immunohistochemistry-Paraffin: dUTPase Antibody (HL1210) - Azide and BSA Free [NBP3-25445] - DUT antibody [HL1210] detects DUT protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human A549. DUT stained by DUT antibody [HL1210] (NBP3-25445) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: dUTPase Antibody (HL1210) - Azide and BSA Free [NBP3-25445] -
Western Blot: dUTPase Antibody (HL1210) - Azide and BSA Free [NBP3-25445] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with DUT antibody [HL1210] (NBP3-25445) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: dUTPase Antibody (HL1210) - Azide and BSA Free [NBP3-25445] -
Immunocytochemistry/Immunofluorescence: dUTPase Antibody (HL1210) - Azide and BSA Free [NBP3-25445] - DUT antibody [HL1210] detects DUT protein at cytoplasm, nucleus and centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DUT stained by DUT antibody [HL1210] (NBP3-25445) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Applications for dUTPase Antibody (HL1210) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: dUTPase
Alternate Names
deoxyuridine triphosphatase, dUTP pyrophosphatasedUTP nucleotidohydrolase, dUTPasedeoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial, EC 3.6.1.23, FLJ20622
Gene Symbol
DUT
Additional dUTPase Products
Product Documents for dUTPase Antibody (HL1210) - Azide and BSA Free
Product Specific Notices for dUTPase Antibody (HL1210) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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