dUTPase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56624

Novus Biologicals, part of Bio-Techne
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NB100-56624
NB100-56624SS
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all dUTPase Antibodies »

Product Summary for dUTPase Antibody

Immunogen

This antibody was developed against a mixture of KLH-conjugated synthetic peptides corresponding to amino acids 25-44 and 113-130 of human dUTPase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for dUTPase Antibody

Western Blot: dUTPase Antibody [NB100-56624]

Western Blot: dUTPase Antibody [NB100-56624]

Western Blot: dUTPase Antibody [NB100-56624] - Detection of DUT-N in HeLa cell lysate using this antibody at a dilution of 1:1000.

Applications for dUTPase Antibody

Application
Recommended Usage

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: dUTPase

Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is a ubiquitous enzyme that functions in the hydrolysis of dUTP to dUMP and pyrophosphate. Canman and co-workers have demonstrated that, in certain human tumor cell lines, increased levels of dUTPase are responsible for an increase in resistance to the cancer chemotherapeutic agent fluorodeoxyuridine (FUdR), a thymidine synthase inhibitor. Two distinct forms of dUTPase exist in humans. Cellular fractionation experiments suggest that the more abundant, lower mass form of dUTPase (DUT-N) localizes in the nucleus, while the higher mass form (DUT-M) is associated with the mitochondria.

Alternate Names

deoxyuridine triphosphatase, dUTP pyrophosphatasedUTP nucleotidohydrolase, dUTPasedeoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial, EC 3.6.1.23, FLJ20622

Entrez Gene IDs

1854 (Human)

Gene Symbol

DUT

UniProt

P33316 (Human)

Additional dUTPase Products

Product Documents for dUTPase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for dUTPase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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