dUTPase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16241

Novus Biologicals, part of Bio-Techne
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NBP2-16241
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all dUTPase Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human dUTPase. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%), Chicken (83%), Bovine (87%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for dUTPase Antibody

Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-16241]

Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-16241]

Immunohistochemistry-Paraffin: dUTPase Antibody [NBP2-16241] - Immunohistochemical analysis of paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.
Immunoprecipitation: dUTPase Antibody [NBP2-16241]

Immunoprecipitation: dUTPase Antibody [NBP2-16241]

Immunoprecipitation: dUTPase Antibody [NBP2-16241] - Immunoprecipitation of DUT protein from A431 whole cell extracts using 5 ug of DUT antibody. Western blot analysis was performed using DUT antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
dUTPase Antibody

Western Blot: dUTPase Antibody [NBP2-16241] -

Western Blot: dUTPase Antibody [NBP2-16241] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with dUTPase antibody (NBP2-16241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for dUTPase Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: dUTPase

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq]

Alternate Names

deoxyuridine triphosphatase, dUTP pyrophosphatasedUTP nucleotidohydrolase, dUTPasedeoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial, EC 3.6.1.23, FLJ20622

Gene Symbol

DUT

Additional dUTPase Products

Product Documents for dUTPase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for dUTPase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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