DUX4 Antibody (P4H2) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49552CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-49552AF350

Alexa Fluor 405

NBP1-49552AF405

Alexa Fluor 488

NBP1-49552AF488

Alexa Fluor 532

NBP1-49552AF532

Alexa Fluor 594

NBP1-49552AF594

Alexa Fluor 647

NBP1-49552AF647

Alexa Fluor 700

NBP1-49552AF700

Alexa Fluor 750

NBP1-49552AF750

Biotin

NBP1-49552B

DyLight 350

NBP1-49552UV

DyLight 405

NBP1-49552V

DyLight 488

NBP1-49552G

DyLight 550

NBP1-49552R

DyLight 594

NBP1-49552DL594

DyLight 650

NBP1-49552C

DyLight 680

NBP1-49552FR

DyLight 755

NBP1-49552IR

FITC

NBP1-49552F

HRP

NBP1-49552H

Janelia Fluor 525

NBP1-49552JF525

Janelia Fluor 549

NBP1-49552JF549

Janelia Fluor 585

NBP1-49552JF585

Janelia Fluor 635

NBP1-49552JF635

Janelia Fluor 646

NBP1-49552JF646

Janelia Fluor 669

NBP1-49552JF669

mFluor Violet 450 SE

NBP1-49552MFV450

mFluor Violet 500 SE

NBP1-49552MFV500

mFluor Violet 610 SE

NBP1-49552MFV610

Forumulation

Catalog #

BSA Free

NBP1-49552

Azide and BSA Free

NBP2-80703

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG₁ Clone # P4H2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DUX4 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the C-terminus of human DUX4.

Specificity

This antibody is specific for DUX4 and does not recognize DUX4c.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for DUX4 Antibody (P4H2) [CoraFluor™ 1]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: DUX4

The DUX4 gene is located within a D4Z4 repeat array in the subtelomeric region of chromosome 4q. The D4Z4 repeat is polymorphic in length; a similar D4Z4 repeat array has been identified on chromosome 10. DUX4 (also known as Double homeobox 4) is the leading candidate causative gene for facioscapulohumeral dystrophy (FSHD), a degenerative skeletal muscle disease and one of the most common muscular dystrophies. FSHD is caused by the deletion of a subset of D4Z4 macrosatellite repeats on chromosome 4. Each repeat contains a retrogene encoding the double-homeobox factor DUX4. DUX4 expression is epigenetically suppressed in differentiated tissues and the residual DUX4 transcripts are spliced to remove the carboxyterminal domain that has been associated with cell toxicity. In FSHD individuals, the expression of the full-length DUX4 transcript is not completely suppressed in skeletal muscle, and possibly other differentiated tissues, and results in a small percentage of cells expressing relatively abundant amounts of the full-length DUX4 mRNA and protein. [Snider et al. (2010) PLoS Genetics.6(10): e1001181]

Long Name

Double Homeobox 4

Alternate Names

DUX10, DUX4L1

Gene Symbol

DUX4

Additional DUX4 Products

Product Documents for DUX4 Antibody (P4H2) [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for DUX4 Antibody (P4H2) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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