DUX4 Antibody (P4H2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80703
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # P4H2
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide corresponding to the C-terminus of human DUX4.
Localization
Nucleus. Note: Actively transported through the nuclear pore complex (NPC)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DUX4 Antibody (P4H2) - Azide and BSA Free
Western Blot: DUX4 Antibody (P4H2)Azide and BSA Free [NBP2-80703]
Western Blot: DUX4 Antibody (P4H2) - Azide and BSA Free [NBP2-80703] - Total protein from human testis tissue and human K562, HeLa and HepG2 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-DUX4 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.Immunocytochemistry/ Immunofluorescence: DUX4 Antibody (P4H2) - Azide and BSA Free [NBP2-80703]
Immunocytochemistry/Immunofluorescence: DUX4 Antibody (P4H2) - Azide and BSA Free [NBP2-80703] - Detection of human DUX4 on C2C12 mouse myoblasts transfected with pCS2-DUX4. Cells were counterstained with DAPI for nuclei. Image from the standard format of this antibody.Immunohistochemistry: DUX4 Antibody (P4H2) - Azide and BSA Free [NBP2-80703]
Immunohistochemistry: DUX4 Antibody (P4H2) - Azide and BSA Free [NBP2-80703] - Analysis of DUX4 on human testis Image from the standard format of this antibody.Applications for DUX4 Antibody (P4H2) - Azide and BSA Free
Application
Recommended Usage
ELISA
reported in scientific literature (PMID 21529284)
Immunocytochemistry/ Immunofluorescence
1:100-1:200
Immunohistochemistry
1:100-1:200
Immunohistochemistry-Frozen
1:100-1:200
Immunohistochemistry-Paraffin
1:100-1:200
Simple Western
1:10
Western Blot
0.5-2.0ug/ml
Application Notes
In Western blot, a band can be seen at approx. 52 kDa. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DUX4
Long Name
Double Homeobox 4
Alternate Names
DUX10, DUX4L1
Gene Symbol
DUX4
Additional DUX4 Products
Product Documents for DUX4 Antibody (P4H2) - Azide and BSA Free
Product Specific Notices for DUX4 Antibody (P4H2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...