DYKDDDDK Epitope Tag Antibody (231)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43570
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Cited:
Epitope Tag
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 231
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The immunogen used to generate this antibody corresponds to DYKDDDDK Epitope Tag
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for DYKDDDDK Epitope Tag Antibody (231)
![Immunoprecipitation: DYKDDDDK Epitope Tag Antibody (231) [NBP2-43570]](https://resources.bio-techne.com/images/products/DYKDDDDK-Epitope-Tag-Antibody-231-Immunoprecipitation-NBP2-43570-img0001.jpg "Immunoprecipitation: DYKDDDDK Epitope Tag Antibody (231) [NBP2-43570]")
Immunoprecipitation: DYKDDDDK Epitope Tag Antibody (231) [NBP2-43570]
Immunoprecipitation: DYKDDDDK Epitope Tag Antibody (231) [NBP2-43570] - Analysis of 750 ug whole cell lysate/extract of DDDK-tagged protein expressing 293T cells. A : 5 ug whole cell lysate/extract of DDDDK-tagged protein expressing 293T cells B : Control with 2.5 ug of pre-immune mouse IgG C : Immunoprecipitation of DDDK-tagged protein by 2.5 ug of DYKDDDDK Epitope Tag Antibody (231) [NBP2-43570] 7.5% SDS-PAGE The immunoprecipitated DDDK-tagged protein was detected by DYKDDDDK Epitope Tag Antibody (231) diluted at 1 : 5000. EasyBlot anti-mouse IgG was used as a secondary reagent.
Western Blot: DYKDDDDK Epitope Tag Antibody (231) [NBP2-43570] -
Western Blot: DYKDDDDK Epitope Tag Antibody (231) [NBP2-43570] - DDDDK Tag antibody [GT231] detects DDDDK Tag protein by Western blot analysis.A. 30 ug 293T whole cell extract
B. 30 ug whole cell extract of DDDDK-tagged protein expressing 293T cells
12 % SDS-PAGE
DDDDK Tag antibody [GT231] (NBP2-43570) dilution: 1:5000
Applications for DYKDDDDK Epitope Tag Antibody (231)
Application
Recommended Usage
Immunoprecipitation
1:100-1:500
Western Blot
1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: DYKDDDDK Epitope Tag
The epitope tag, DYKDDDDK, is comprised of an eight amino acid sequence derived from the gene-10 product from bacteriophage T7 and contains an enterokinase-cleavage site (1). Its hydrophilic nature and short peptide sequence reduce possible interference with protein expression, post-translational modifications, protein interactions, and protein function (2). The DYKDDDDK (FLAG(R)) epitope tag can either be fused to the N-terminus, C-terminus, or placed at an internal position of the target protein, facilitating characterization of the protein of interest using immunodetection methods. Compared to the standard DYKDDDDK epitope tag antibody, the rat monoclonal DYKDDDDK Epitope Tag antibody (clone L5) is 2-8 times more sensitive (3).
References
1. Brizzard BL, Chubet RG, Vizard DL. (1994) Immunoaffinity purification of (FLAG(R)) epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques. 16(4):730-5. PMID: 8024796
2. Einhauer A, Jungbauer A. (2001) The (FLAG(R)) peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 49(1-3):455-65. PMID: 11694294
3. Park SH, Cheong C, Idoyaga J, Kim JY, Choi JH, Do Y, Lee H, Jo JH, Oh YS, Im W, Steinman RM, Park CG. (2008) Generation and application of new rat monoclonal antibodies against synthetic DYKDDDDK Epitope Tag (FLAG(R)) and OLLAS tags for improved immunodetection. J Immunol Methods. 331(1-2):27-38. PMID: 18054954
FLAG(R) is a trademark of Sigma-Aldrich.
Alternate Names
FLAG Tag
Additional DYKDDDDK Epitope Tag Products
Product Documents for DYKDDDDK Epitope Tag Antibody (231)
Product Specific Notices for DYKDDDDK Epitope Tag Antibody (231)
FLAG(R) and ANTI-FLAG(R) are registered trademarks of Sigma-Aldrich Co. LLC.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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