DYKDDDDK Epitope Tag Antibody (2F11G1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37680
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 2F11G1
Format
BSA Free
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Summary for DYKDDDDK Epitope Tag Antibody (2F11G1) - BSA Free
Immunogen
Synthetic peptide corresponding to aa(DYKDDDDK), conjugated to KLH.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
1.01 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DYKDDDDK Epitope Tag Antibody (2F11G1) - BSA Free
![Western Blot: DYKDDDDK Epitope Tag Antibody (2F11G1)BSA Free [NBP2-37680]](https://resources.bio-techne.com/images/products/DYKDDDDK-Epitope-Tag-Antibody-2F11G1-Western-Blot-NBP2-37680-img0002.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody (2F11G1)BSA Free [NBP2-37680]")
Western Blot: DYKDDDDK Epitope Tag Antibody (2F11G1)BSA Free [NBP2-37680]
Western Blot: DYKDDDDK Epitope Tag Antibody (2F11G1) [NBP2-37680] - Analysis using mouse mAb DYKDDDDK Epitope Tag Antibody (2F11G1) [NBP2-37680] against two different fusion protein (1), (2) with flag tag.Applications for DYKDDDDK Epitope Tag Antibody (2F11G1) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Western Blot
1:500- 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Format
BSA Free
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DYKDDDDK Epitope Tag
The epitope tag, DYKDDDDK, is comprised of an eight amino acid sequence derived from the gene-10 product from bacteriophage T7 and contains an enterokinase-cleavage site (1). Its hydrophilic nature and short peptide sequence reduce possible interference with protein expression, post-translational modifications, protein interactions, and protein function (2). The DYKDDDDK (FLAG(R)) epitope tag can either be fused to the N-terminus, C-terminus, or placed at an internal position of the target protein, facilitating characterization of the protein of interest using immunodetection methods. Compared to the standard DYKDDDDK epitope tag antibody, the rat monoclonal DYKDDDDK Epitope Tag antibody (clone L5) is 2-8 times more sensitive (3).
References
1. Brizzard BL, Chubet RG, Vizard DL. (1994) Immunoaffinity purification of (FLAG(R)) epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques. 16(4):730-5. PMID: 8024796
2. Einhauer A, Jungbauer A. (2001) The (FLAG(R)) peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 49(1-3):455-65. PMID: 11694294
3. Park SH, Cheong C, Idoyaga J, Kim JY, Choi JH, Do Y, Lee H, Jo JH, Oh YS, Im W, Steinman RM, Park CG. (2008) Generation and application of new rat monoclonal antibodies against synthetic DYKDDDDK Epitope Tag (FLAG(R)) and OLLAS tags for improved immunodetection. J Immunol Methods. 331(1-2):27-38. PMID: 18054954
FLAG(R) is a trademark of Sigma-Aldrich.
Alternate Names
FLAG Tag
Additional DYKDDDDK Epitope Tag Products
Product Documents for DYKDDDDK Epitope Tag Antibody (2F11G1) - BSA Free
Product Specific Notices for DYKDDDDK Epitope Tag Antibody (2F11G1) - BSA Free
FLAG(R) and ANTI-FLAG(R) are registered trademarks of Sigma-Aldrich Co. LLC.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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