DYKDDDDK Epitope Tag Horseradish Peroxidase-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # HAM85291
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Human
Applications
Validated:
Western Blot
Cited:
ELISA Detection
Label
HRP
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1042E
Product Specifications
Immunogen
DYKDDDDK epitope tag
Specificity
Detects N-terminal and C-terminal DYKDDDDK (FLAG®) epitope tagged proteins in Western blot.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DYKDDDDK Epitope Tag Horseradish Peroxidase-conjugated Antibody
Detection of DYKDDDDK (FLAG®) Epitope Tag by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1 and N-terminal DYKDDDDK-tagged RNF20. PVDF membrane was probed with 1:1000-1:3000 dilution of Rabbit Anti-DYKDDDDK Epitope Tag HRP-conjugated Monoclonal Antibody (Catalog # HAM85291). Specific bands were detected for DYKDDDDK-tagged proteins at approximately 55 kDa and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for DYKDDDDK Epitope Tag Horseradish Peroxidase-conjugated Antibody
Application
Recommended Usage
Western Blot
1:1000-1:3000 dilution
Sample: HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1 and N-terminal DYKDDDDK-tagged RNF20
Sample: HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1 and N-terminal DYKDDDDK-tagged RNF20
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Formulation
Supplied as a 0.2 μm filtered solution in PBS
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: DYKDDDDK Epitope Tag
The epitope tag, DYKDDDDK, is comprised of an eight amino acid sequence derived from the gene-10 product from bacteriophage T7 and contains an enterokinase-cleavage site (1). Its hydrophilic nature and short peptide sequence reduce possible interference with protein expression, post-translational modifications, protein interactions, and protein function (2). The DYKDDDDK (FLAG(R)) epitope tag can either be fused to the N-terminus, C-terminus, or placed at an internal position of the target protein, facilitating characterization of the protein of interest using immunodetection methods. Compared to the standard DYKDDDDK epitope tag antibody, the rat monoclonal DYKDDDDK Epitope Tag antibody (clone L5) is 2-8 times more sensitive (3).
References
1. Brizzard BL, Chubet RG, Vizard DL. (1994) Immunoaffinity purification of (FLAG(R)) epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques. 16(4):730-5. PMID: 8024796
2. Einhauer A, Jungbauer A. (2001) The (FLAG(R)) peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 49(1-3):455-65. PMID: 11694294
3. Park SH, Cheong C, Idoyaga J, Kim JY, Choi JH, Do Y, Lee H, Jo JH, Oh YS, Im W, Steinman RM, Park CG. (2008) Generation and application of new rat monoclonal antibodies against synthetic DYKDDDDK Epitope Tag (FLAG(R)) and OLLAS tags for improved immunodetection. J Immunol Methods. 331(1-2):27-38. PMID: 18054954
FLAG(R) is a trademark of Sigma-Aldrich.
Alternate Names
FLAG Tag
Additional DYKDDDDK Epitope Tag Products
Product Documents for DYKDDDDK Epitope Tag Horseradish Peroxidase-conjugated Antibody
Product Specific Notices for DYKDDDDK Epitope Tag Horseradish Peroxidase-conjugated Antibody
FLAG is a registered trademark of SigmaAldrich Co. LLC
For research use only
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