Dynactin Subunit 1/DCTN1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30880

Novus Biologicals, part of Bio-Techne
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NBP1-30880
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Dynactin Subunit 1/DCTN1 Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Dynactin Subunit 1/DCTN1. The exact sequence is proprietary.

Localization

Cytoplasm, cytoskeleton

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

142 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dynactin Subunit 1/DCTN1 Antibody

Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880]

Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880]

Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DCTN1 antibody [C3], C-term (NBP1-30880). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880]

Immunocytochemistry/ Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880]

Immunocytochemistry/Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880] - Analysis of methanol-fixed HeLa, using DCTN1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880]

Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880]

Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880] - Paraffin-embedded Rat brain. DCTN1 antibody [C3], C-term diluted at 1:500.
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Applications for Dynactin Subunit 1/DCTN1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7)

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dynactin Subunit 1/DCTN1

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein. Alternative splicing of this gene results in at least 2 functionally distinct isoforms: a ubiquitously expressed one and a brain-specific one. Based on its cytogenetic location, this gene is considered as a candidate gene for limb-girdle muscular dystrophy.

Alternate Names

DAP-150, DCTN1, DP-150, Dynactin 1, HMN7B

Gene Symbol

DCTN1

Additional Dynactin Subunit 1/DCTN1 Products

Product Documents for Dynactin Subunit 1/DCTN1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Dynactin Subunit 1/DCTN1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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