Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Dynactin Subunit 1/DCTN1 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1228 to 1278 of human 150 kDa Dynein-Associated Polypeptide using the numbering given in entry NP_004073.2 (GeneID 1639).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dynactin Subunit 1/DCTN1 Antibody

Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NBP1-71829]

Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NBP1-71829]

Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NBP1-71829] - Whole cell lysate from Jurkat (5, 15 and 50 mcg for WB), HeLa (H, 50 mcg; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-p150glued antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. p150glued was also immunoprecipitated by rabbit anti-p150glued antibody NBP1-71828, which recognizes an upstream epitope.

Applications for Dynactin Subunit 1/DCTN1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Dynactin Subunit 1/DCTN1

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein. Alternative splicing of this gene results in at least 2 functionally distinct isoforms: a ubiquitously expressed one and a brain-specific one. Based on its cytogenetic location, this gene is considered as a candidate gene for limb-girdle muscular dystrophy.

Alternate Names

DAP-150, DCTN1, DP-150, Dynactin 1, HMN7B

Gene Symbol

DCTN1

UniProt

NP_004073.2 (Human)

Additional Dynactin Subunit 1/DCTN1 Products

Product Documents for Dynactin Subunit 1/DCTN1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Dynactin Subunit 1/DCTN1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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