Dynamin Antibody (3G4B6)

Immunoprecipitation: Dynamin Antibody (3G4B6) [NB110-60491]

Immunoprecipitation: Dynamin Antibody (3G4B6) [NB110-60491] - Antibody-bead conjugates were prepared by adding 1.0 ug of antibody to 500 ul of PBS with 0,01% triton X-100 in a microcentrifuge tube and 30ul of protein G Sepharose beads. Tubes were rocked O/N at 4 degrees C followed by several washes to remove unbound antibodies. U2OS were collected in HEPES buffer (20 mM HEPES, 100 mM sodium chloride, 1 mM EDTA, 1% Triton X-100, pH 7.4) supplemented with protease inhibitor. Lysates are rocked 30 min at 4 degrees C and spun at 110,000xg for 15 min at 4 degrees C. One ml aliquots at 1 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4 degrees C. Following centrifugation, the unbound fractions were collected, and beads were subsequently washed three times with 1 ml of HEPES lysis buffer and processed for SDS-PAGE and immunoblot. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Western Blot: Dynamin Antibody (3G4B6) [NB110-60491]

Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot analysis using anti-Dynamin monoclonal antibody against truncated Dynamin recombinant protein (1), SK-N-SH (2) and NIH3T3 (3) cell lysate.

Immunohistochemistry-Paraffin: Dynamin Antibody (3G4B6) [NB110-60491]

Immunohistochemistry-Paraffin: Dynamin Antibody (3G4B6) [NB110-60491] - Immunohistochemical analysis of paraffin-embedded human Lymph tissue, glioma tissue and cerebellum tissue, showing membrane localization using Dynamin antibody with DAB staining.

Flow Cytometry: Dynamin Antibody (3G4B6) [NB110-60491]

Flow Cytometry: Dynamin Antibody (3G4B6) [NB110-60491] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dynamin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Knockout Validated: Dynamin Antibody (3G4B6) [NB110-60491]

Knockout Validated: Dynamin Antibody (3G4B6) [NB110-60491] - U2OS WT and DNM1 KO cells were labelled with a green or a far red fluorescence dye, respectively. WT and KO cells were mixed and plated to a 1 :1 ratio on coverslips. Cells were stained with and with the corresponding Alexa-fluor 555 coupled secondary antibody. Acquisition of the green (WT), red (antibody staining) and far-red (KO) channels was performed. Representative grayscale image of the red channel is shown. WT and KO cells are outlined with yellow and magenta dashed line, respectively. Antibody dilution used: 1/1000. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).