Dynamin Antibody (D5)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1993

Novus Biologicals, part of Bio-Techne
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NB100-1993
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Chicken

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # D5

Concentration

1.0 mg/ml

View all Dynamin Antibodies »

Product Summary for Dynamin Antibody (D5)

Immunogen

Recombinant His6-tagged human dynamin.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Microtubule associated.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Dynamin Antibody (D5)

Western Blot: Dynamin Antibody (D5) [NB100-1993]

Western Blot: Dynamin Antibody (D5) [NB100-1993]

Western Blot: Dynamin Antibody (D5) [NB100-1993] - Analysis of chicken SL29 cell Lysate
Western Blot: Dynamin Antibody (D5) [NB100-1993]

Western Blot: Dynamin Antibody (D5) [NB100-1993]

Western Blot: Dynamin Antibody (D5) [NB100-1993] - Western blot analysis of Dynamin, mAb (D5): Lane 1: MW marker, Lane 2: Mouse Brain, Lane 3: Rat Brain

Applications for Dynamin Antibody (D5)

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

1:10-1:500

Western Blot

0.5 ug/mL
Application Notes
Detects a band of ~100 kDa by Western blot; recommended dilutions for colorimetric detection.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2), 0.1mM PMSF and 50% Glycerol

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dynamin

Dynamin is a 100 kDa GTP binding protein that was first identified as a protein able to bind microtubules (1). A mutation in the Drosophila gene shits, encoding a dynamin homologue, prevents synaptic vesicle recycling and causes temperature-sensitive paralysis, implicating dynamin in the formation of coated vesicles (2). Dynamin may mediate vesicle formation by assembling into a ring structure around the necks of invaginated coated pits that may eventually lead to the closure of the neck and formation of vesicles (3). Amphiphysin, dynamin, clathrin and AP2 form a complex during synaptic vesicle endocytosis, which is regulated by the phosphorylated state of different components in this complex (4). To date, three genes have been found to encode dynamin isoforms. Dynamin 1 is neuronal specific, Dynamin 2 is ubiquitously expressed and Dynamin 3 localizes to testis, brain and lung (5).

Alternate Names

DNM1

Entrez Gene IDs

1759 (Human); 13429 (Mouse); 140694 (Rat)

Gene Symbol

DNM1

UniProt

Q05193 (Human)

Additional Dynamin Products

Product Documents for Dynamin Antibody (D5)

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Product Specific Notices for Dynamin Antibody (D5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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