Dynamin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61094

Novus Biologicals, part of Bio-Techne
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NB100-61094
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chicken (100%), Mouse (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Dynamin Antibodies »

Product Summary for Dynamin Antibody

Immunogen

Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # Q05193

Reactivity Notes

It is also anticipated that the antibody will work with human, mouse, non-human primates, cow and chicken based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Specificity

Specific for endogenous levels of the ~95 kDa dynamin protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dynamin Antibody

Western Blot: Dynamin Antibody [NB100-61094]

Western Blot: Dynamin Antibody [NB100-61094]

Western Blot: Dynamin Antibody [NB100-61094] - Western Blot of rat cortex lysate using NB100-61094 at 1:1000.
Western Blot: Dynamin Antibody [NB100-61094]

Western Blot: Dynamin Antibody [NB100-61094]

Western Blot: Dynamin Antibody [NB100-61094] - Rat cortex lysate showing labeling of the ~95k Dynamin protein.

Applications for Dynamin Antibody

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dynamin

Dynamin is a GTPase required for endocytosis of clathrin coated vesicles from the membrane. Dynamin's involvement in endocytosis was first discovered during the characterization of the temperature sensitive parylytic shibire mutation from Drosophila melanogaster. Three closely related isoforms have been identified in mammals. Dynamin1 is expressed exclusively in neurons, dynamin2 is ubiquitously expressed and dynamin3 is expressed primarily in the testes. Each of these isoforms of dynamin also has several splice variants. This suggests that each isoform/splice variant may have separate intracellular targets or function in the budding of distinct vesicle types. Common domain features of the dynamin proteins include an amino acid terminal GTPase domain, a central pleckstrin homology domain and a highly basic proline rich carboxyl terminus with several Src homology 3 (SH3) domains. These SH3 domains target the dynamin to the clathrin coated pit. Dynamin is known to interact with at least two proteins, AP2 adaptor protein complex and amphiphysin.

Alternate Names

DNM1

Entrez Gene IDs

1759 (Human); 140694 (Rat)

Gene Symbol

DNM1

UniProt

Q05193 (Human)

Additional Dynamin Products

Product Documents for Dynamin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Dynamin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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