Dynamin [p Ser774] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-209

Novus Biologicals, part of Bio-Techne
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NB300-209
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Mouse (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Dynamin Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 conjugated to KLH. Accession # P21575

Modification

p Ser774

Localization

Cytoplasmic

Specificity

Specific for endogenous levels of the ~95 kDa dynamin protein phosphorylated at Ser774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by lambda-phosphatase treatment.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dynamin [p Ser774] Antibody

Western Blot: Dynamin [p Ser774] Antibody [NB300-209]

Western Blot: Dynamin [p Ser774] Antibody [NB300-209]

Western Blot: Dynamin [p Ser774] Antibody [NB300-209] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser774 dynamin. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Applications for Dynamin [p Ser774] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000
Application Notes
NB300-209 can be used in Western blot where a band is seen at ~ 97 kD, representing Dynamin
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dynamin

Dynamins are a family of GTPases transcribed from at least three separate genes. They contain several conserved regions; an amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a region located in front of the proline-rich domain (PRD). This PRD region is thought to mediate interactions between dynamin and other cellular proteins. There are also at least three isoforms of this protein. Dynamin 2 is ubiquitous. Dynamin 3 is expressed in the brain, testis, heart and lung. Dynamin 1 is exclusively neuronal and is phosphorylated by PKC and dephosphorylated by calcineurin during an action potential in the nerve terminal. It is believed that this dephosphorylation provides a trigger for the cellular process of clatherin-mediated and fluid-phase endocytosis.

Alternate Names

DNM1

Entrez Gene IDs

1759 (Human); 140694 (Rat)

Gene Symbol

DNM1

UniProt

P21575 (Rat)

Additional Dynamin Products

Product Documents for Dynamin [p Ser774] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Dynamin [p Ser774] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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