DYNC1I2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38469

Novus Biologicals, part of Bio-Techne
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NBP2-38469
NBP2-38469-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DYNC1I2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: HSDSDLGRGPIKLGMAKITQVDFPPREIVTYTKETQTPVMAQPKEDEEE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DYNC1I2 Antibody

Western Blot: DYNC1I2 Antibody [NBP2-38469]

Western Blot: DYNC1I2 Antibody [NBP2-38469]

Western Blot: DYNC1I2 Antibody [NBP2-38469] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: DYNC1I2 Antibody [NBP2-38469]

Immunocytochemistry/ Immunofluorescence: DYNC1I2 Antibody [NBP2-38469]

Immunocytochemistry/Immunofluorescence: DYNC1I2 Antibody [NBP2-38469] - Staining of human cell line HEK 293 shows positivity in cytoplasm and aggresome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DYNC1I2 Antibody [NBP2-38469]

Immunohistochemistry-Paraffin: DYNC1I2 Antibody [NBP2-38469]

Immunohistochemistry-Paraffin: DYNC1I2 Antibody [NBP2-38469] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
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Applications for DYNC1I2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DYNC1I2

The intermediate chains seem to help dynein bind to dynactin 150 kDa component

Alternate Names

cytoplasmic dynein 1 intermediate chain 2, Cytoplasmic dynein intermediate chain 2, DH IC-2, DNCI2MGC104199, DNCIC2, Dynein intermediate chain 2, cytosolic, dynein, cytoplasmic 1, intermediate chain 2, dynein, cytoplasmic, intermediate polypeptide 2, FLJ21089, FLJ90842, IC2, MGC111094, MGC9324

Gene Symbol

DYNC1I2

UniProt

Q13409 (Human)

Additional DYNC1I2 Products

Product Documents for DYNC1I2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DYNC1I2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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