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DYNC1I2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38714

Novus Biologicals, part of Bio-Techne
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NBP2-38714
NBP2-38714-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: EDDDVVAPKPPIEPEEEKTLKKDEENDSKAPPHELTE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DYNC1I2 Antibody

Western Blot: DYNC1I2 Antibody [NBP2-38714]

Western Blot: DYNC1I2 Antibody [NBP2-38714]

Western Blot: DYNC1I2 Antibody [NBP2-38714] - Analysis using Anti-DYNC1I2 antibody NBP2-38714 (A) shows similar pattern to independent antibody NBP2-38469 (B).
Immunocytochemistry/ Immunofluorescence: DYNC1I2 Antibody [NBP2-38714]

Immunocytochemistry/ Immunofluorescence: DYNC1I2 Antibody [NBP2-38714]

Immunocytochemistry/Immunofluorescence: DYNC1I2 Antibody [NBP2-38714] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center, cytosol & endoplasmic reticulum.
Immunohistochemistry-Paraffin: DYNC1I2 Antibody [NBP2-38714]

Immunohistochemistry-Paraffin: DYNC1I2 Antibody [NBP2-38714]

Immunohistochemistry-Paraffin: DYNC1I2 Antibody [NBP2-38714] - Staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.

Applications for DYNC1I2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DYNC1I2

The intermediate chains seem to help dynein bind to dynactin 150 kDa component

Alternate Names

cytoplasmic dynein 1 intermediate chain 2, Cytoplasmic dynein intermediate chain 2, DH IC-2, DNCI2MGC104199, DNCIC2, Dynein intermediate chain 2, cytosolic, dynein, cytoplasmic 1, intermediate chain 2, dynein, cytoplasmic, intermediate polypeptide 2, FLJ21089, FLJ90842, IC2, MGC111094, MGC9324

Gene Symbol

DYNC1I2

UniProt

Additional DYNC1I2 Products

Product Documents for DYNC1I2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DYNC1I2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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