Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # DMD/3244

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Dystrophin Antibodies »

Product Specifications

Immunogen

A recombinant fragment (around aa 114-263) of human DMD protein (exact sequence is proprietary) (Uniprot: P11532)

Localization

Cell Surface and Cytoplasmic

Marker

Marker of Duchenne and Becker Muscular Dystrophy

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

427 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07653).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Dystrophin Antibody (DMD/3244) - Azide and BSA Free

Immunohistochemistry-Paraffin: Dystrophin Antibody (DMD/3244) - Azide and BSA Free [NBP3-08702]

Immunohistochemistry-Paraffin: Dystrophin Antibody (DMD/3244) - Azide and BSA Free [NBP3-08702]

Immunohistochemistry-Paraffin: Dystrophin Antibody (DMD/3244) [NBP3-08702] - Formalin-fixed, paraffin-embedded human Skeletal Muscle stained with Dystrophin Monospecific Mouse Monoclonal Antibody (DMD/3244)
Protein Array: Dystrophin Antibody (DMD/3244) - Azide and BSA Free [NBP3-08702]

Protein Array: Dystrophin Antibody (DMD/3244) - Azide and BSA Free [NBP3-08702]

Protein Array: Dystrophin Antibody (DMD/3244) [NBP3-08702] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Dystrophin Monospecific Mouse Monoclonal Antibody (DMD/3244).

Applications for Dystrophin Antibody (DMD/3244) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Dystrophin

Dystrophin is a muscle membrane protein (427 kDa) which is absent, reduced or altered as a result of mutation in Duchenne and Becker muscular dystrophies (DMD/BMD) or its homologue in the mouse.8 Severe DMD is associated with a marked dystrophin deficiency whereas patients with the milder form of BMD show less pronounced abnormalities of protein expression. Because abnormalities in the protein expression occur specifically in patients with these types of muscular dystrophy, dystrophin analysis may be used to distinguish these conditions from other neuromuscular diseases. Predictions from the sequence suggest a structural protein on the inner face of the membrane, consisting of a 25-repeat, rod-like triple-helical domain separating an N-terminal actin-binding domain from two C-terminal domains, one of which is rich in cysteine.9 The large size of dystrophin and its low abundance (<0.01% of the total muscle protein) are a hindrance to the isolation of intact, native protein for structure/function studies. Monoclonal antibodies against defined regions10 of dystrophin provide a means for studying its structure and function, interactions with other proteins and the nature of the partial gene products produced in some patients carrying deletions in the dystrophin gene. The antibodies are useful in the prenatal or post-abortion diagnosis of muscular dystrophy carriers by immunohistological analyses.11

Alternate Names

BMDDXS272, CMD3B, DXS142, DXS164, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, dystrophin, dystrophin (muscular dystrophy, Duchenne and Becker types), includes DXS142

Gene Symbol

DMD

Additional Dystrophin Products

Product Documents for Dystrophin Antibody (DMD/3244) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Dystrophin Antibody (DMD/3244) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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