E-Cadherin Antibody (rCDH1/6769) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23977
Recombinant Monoclonal Antibody
![E-Cadherin Antibody (rCDH1/6769) - Azide and BSA Free Immunohistochemistry-Paraffin: E-Cadherin Antibody (rCDH1/6769) - Azide and BSA Free [NBP3-23977] -](https://resources.bio-techne.com/images/products/nbp3-23977_mouse-e-cadherin-mab-rcdh1-6769-azide-and-bsa-free-241202580164.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (rCDH1/6769) - Azide and BSA Free [NBP3-23977] -")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # rCDH1/6769
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa600-700) of human E-Cadherin (exact sequence is proprietary)
Localization
Cell Surface
Marker
Intercellular Junction Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Antibody without azide - store at -20 to -80C.
Scientific Data Images for E-Cadherin Antibody (rCDH1/6769) - Azide and BSA Free
Immunohistochemistry-Paraffin: E-Cadherin Antibody (rCDH1/6769) - Azide and BSA Free [NBP3-23977] -
Immunohistochemistry-Paraffin: E-Cadherin Antibody (rCDH1/6769) - Azide and BSA Free [NBP3-23977] - Formalin-fixed, paraffin-embedded human colon stained with E-Cadherin Antibody (rCDH1/6769). Inset: PBS instead of primary antibody; secondary only negative control.![E-Cadherin Antibody (rCDH1/6769) - Azide and BSA Free Immunohistochemistry-Paraffin: E-Cadherin Antibody (rCDH1/6769) - Azide and BSA Free [NBP3-23977] -](https://resources.bio-techne.com/images/products/nbp3-23977_mouse-e-cadherin-mab-rcdh1-6769-azide-and-bsa-free-24120257592853.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (rCDH1/6769) - Azide and BSA Free [NBP3-23977] -")
Immunohistochemistry-Paraffin: E-Cadherin Antibody (rCDH1/6769) - Azide and BSA Free [NBP3-23977] -
Immunohistochemistry-Paraffin: E-Cadherin Antibody (rCDH1/6769) - Azide and BSA Free [NBP3-23977] - IHC analysis of formalin-fixed, paraffin-embedded human stomach. Stained using E-Cadherin Antibody (rCDH1/6769) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.Applications for E-Cadherin Antibody (rCDH1/6769) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: E-Cadherin
Alternate Names
Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin
Gene Symbol
CDH1
Additional E-Cadherin Products
Product Documents for E-Cadherin Antibody (rCDH1/6769) - Azide and BSA Free
Product Specific Notices for E-Cadherin Antibody (rCDH1/6769) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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