E2F-2 Antibody (1H7D6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16165
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1H7D6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 303-395 of human E2F-2 (Q14209). CPEEVQEPDSPSEEPLPSTSTLCPSPDSAQPSSSTDPSIMEPTASSVPAPAPTPQQAPPPPSLVPLEATDSLLELPHPLLQQTEDQFLSPTLA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for E2F-2 Antibody (1H7D6)
![Western Blot: E2F-2 Antibody (1H7D6) [NBP3-16165]](https://resources.bio-techne.com/images/products/E2F-2-Antibody-1H7D6-Western-Blot-NBP3-16165-img0001.jpg "Western Blot: E2F-2 Antibody (1H7D6) [NBP3-16165]")
Western Blot: E2F-2 Antibody (1H7D6) [NBP3-16165]
Western Blot: E2F-2 Antibody (1H7D6) [NBP3-16165] - Western blot analysis of extracts of various cell lines, using E2F-2 Rabbit mAb (NBP3-16165) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.Applications for E2F-2 Antibody (1H7D6)
Application
Recommended Usage
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: E2F-2
Long Name
E2F Transcription Factor 2
Alternate Names
E2F2
Gene Symbol
E2F2
Additional E2F-2 Products
Product Documents for E2F-2 Antibody (1H7D6)
Product Specific Notices for E2F-2 Antibody (1H7D6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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