Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Canine (100%), Equine (100%), Guinea Pig (100%), Monkey (100%), Primate (100%), Rabbit (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all E6AP/UBE3A Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a portion of human ubiquitin protein ligase E3A encoded within exon 6 (LocusLink ID 7337).

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, White-tufted-ear marmoset, Crabeating macaque, Naked mole rat, Thirteen-lined ground squirrel and Northern white-cheeked gibbon.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for E6AP/UBE3A Antibody

Western Blot: E6AP/UBE3A Antibody [NB500-239]

Western Blot: E6AP/UBE3A Antibody [NB500-239]

Western Blot: E6AP/UBE3A Antibody [NB500-239] - Detection of Human and Mouse E6AP by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-E6AP antibody NB500-239 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: E6AP/UBE3A Antibody [NB500-239]

Western Blot: E6AP/UBE3A Antibody [NB500-239]

Western Blot: E6AP/UBE3A Antibody [NB500-239] - Cytoplasmic (S100) fraction (10 mg) from HeLa cells. Antibody used at 20 ug/10 mg extract.

Applications for E6AP/UBE3A Antibody

Application
Recommended Usage

Immunoprecipitation

1 - 4 ug/mg of lysate

Western Blot

1:1000 - 1:5000
Application Notes
It recognizes a band at ~104 kDa in Western blot, representing E6AP (in cytosolic or nuclear extracts). *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: E6AP/UBE3A

E6AP was originally identified as the ubiquitin-protein ligase involved in human papillomavirus E6-mediated p53 degradation. It has since been shown to act as an E3 ubiquitin-protein ligase catalyzing the ubiquitination of several other protein substrates. Ubiquitin E3 ligases are overexpressed in many human cancers and their inhibition leads to growth suppression or apoptosis.

Long Name

Human Papillomavirus E6-associated Protein

Alternate Names

ANCR, EPVE6AP, HPVE6A, UBE3A

Entrez Gene IDs

7337 (Human)

Gene Symbol

UBE3A

Additional E6AP/UBE3A Products

Product Documents for E6AP/UBE3A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for E6AP/UBE3A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.