E74 like factor 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1069

Novus Biologicals, part of Bio-Techne
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NB100-1069
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all E74 like factor 1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-AMKQNELLEPNSF corresponding to C-Terminus according to NP_758961.1, NP_001138825.1.

Localization

Nuclear

Specificity

This antibody is expected to recognise both reported isoforms.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for E74 like factor 1 Antibody

Western Blot: E74 like factor 1 Antibody [NB100-1069]

Western Blot: E74 like factor 1 Antibody [NB100-1069]

Western Blot: E74 like factor 1 Antibody [NB100-1069] - (0.3ug/ml) staining of Human Lymph Node (A), Human Tonsil (B) and Mouse Thymus (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: E74 like factor 1 Antibody [NB100-1069]

Immunohistochemistry-Paraffin: E74 like factor 1 Antibody [NB100-1069]

Immunohistochemistry-Paraffin: E74 like factor 1 Antibody [NB100-1069] - Staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: E74 like factor 1 Antibody [NB100-1069]

Western Blot: E74 like factor 1 Antibody [NB100-1069]

Western Blot: E74 like factor 1 Antibody [NB100-1069] - (0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for E74 like factor 1 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.03 - 0.1 ug/ml
Application Notes
Approx 65kDa band observed in Human Lymph Node and Tonsil and in Mouse Thymus lysates (calculated MW of 64.9kDa according to Human NP_001138825.1 and 62.3kDa according to Mouse NP_001273341.1).
Approx 70kDa band observed in lysates of cell line Jurkat (calculated MW of 67.5kDa according to NP_758961.1).
IHC: Paraffin embedded Human Thymus.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: E74 like factor 1

E74-like factor 1 (ELF1) is a member of the ETS (e26 transformation specific sequence) family of proteins, contains an ETS-domain, and shares sequence identity with the Drosophila ecdysone inducible E74 transcription factor. ETS was originally identified in the E26 retroviral isolate that led to the discovery of c-ets-1 (cellular ets-1), a cellular oncogenic transcription factor capable of transforming cells. Like ETS, ELF-1 functions as a transcription factor and has been shown to bind promoter and enhancer elements in a number of immunity responsive genes. ELF-1 is highly expressed in B-cells and appears to play an integral role in B-cell gene expression.

Alternate Names

E74-like factor 1, E74-like factor 1 (ets domain transcription factor), ETS-related transcription factor Elf-1

Entrez Gene IDs

1997 (Human); 13709 (Mouse)

Gene Symbol

ELF1

UniProt

NP_758961. (Human)

Additional E74 like factor 1 Products

Product Documents for E74 like factor 1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for E74 like factor 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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