E74 like factor 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90352

Novus Biologicals, part of Bio-Techne
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NBP1-90352
NBP1-90352-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all E74 like factor 1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PSSSIESSDPSLSSSATSNRNQTSRSRVSSSPGVKGGATTVLKPGNSKAAKPKDPVEVAQPSEVLRTVQPTQSPYPTQLFRTVHVVQPVQAVPEGEAARTSTMQDETLNSSVQSIRTIQAPTQVPVVVSPRNQQL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for E74 like factor 1 Antibody

Western Blot: E74 like factor 1 Antibody [NBP1-90352]

Western Blot: E74 like factor 1 Antibody [NBP1-90352]

Western Blot: E74 like factor 1 Antibody [NBP1-90352] - Analysis in human cell line HEL.
Immunohistochemistry-Paraffin: E74 like factor 1 Antibody [NBP1-90352]

Immunohistochemistry-Paraffin: E74 like factor 1 Antibody [NBP1-90352]

Immunohistochemistry-Paraffin: E74 like factor 1 Antibody [NBP1-90352] - Staining of human lymph node shows distinct nuclear positivity in germinal center cells.
Western Blot: E74 like factor 1 Antibody [NBP1-90352]

Western Blot: E74 like factor 1 Antibody [NBP1-90352]

Western Blot: E74 like factor 1 Antibody [NBP1-90352] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Applications for E74 like factor 1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: E74 like factor 1

E74-like factor 1 (ELF1) is a member of the ETS (e26 transformation specific sequence) family of proteins, contains an ETS-domain, and shares sequence identity with the Drosophila ecdysone inducible E74 transcription factor. ETS was originally identified in the E26 retroviral isolate that led to the discovery of c-ets-1 (cellular ets-1), a cellular oncogenic transcription factor capable of transforming cells. Like ETS, ELF-1 functions as a transcription factor and has been shown to bind promoter and enhancer elements in a number of immunity responsive genes. ELF-1 is highly expressed in B-cells and appears to play an integral role in B-cell gene expression.

Alternate Names

E74-like factor 1, E74-like factor 1 (ets domain transcription factor), ETS-related transcription factor Elf-1

Gene Symbol

ELF1

Additional E74 like factor 1 Products

Product Documents for E74 like factor 1 Antibody

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Certificate of Analysis

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Product Specific Notices for E74 like factor 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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