EAAT2/GLT1 Antibody (1D8)

Novus Biologicals, part of Bio-Techne | Catalog # H00006506-M07

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1D8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EAAT2/GLT1 Antibodies »

Product Specifications

Immunogen

SLC1A2 (NP_004162, 160 a.a. ~ 239 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DEVSSLDAFLDLIRNLFPENLVQACFQQIQTVTKKVLVAPPPDEEANATSAVVSLLNETVTEVPEETKMVIKKGLEFKDG

Specificity

SLC1A2 - solute carrier family 1 (glial high affinity glutamate transporter), member 2 (1D8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for EAAT2/GLT1 Antibody (1D8)

Western Blot: EAAT2/GLT1 Antibody (1D8) [H00006506-M07]

Western Blot: EAAT2/GLT1 Antibody (1D8) [H00006506-M07]

Western Blot: EAAT2/GLT1 Antibody (1D8) [H00006506-M07] - Analysis of SLC1A2 expression in HeLa (Cat # L013V1).
ELISA: EAAT2/GLT1 Antibody (1D8) [H00006506-M07]

ELISA: EAAT2/GLT1 Antibody (1D8) [H00006506-M07]

ELISA: EAAT2/GLT1 Antibody (1D8) [H00006506-M07] - Detection limit for recombinant GST tagged SLC1A2 is approximately 0.03ng/ml as a capture antibody.

Applications for EAAT2/GLT1 Antibody (1D8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EAAT2/GLT1

This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq]

Long Name

Excitatory Amino Acid Transporter 2

Alternate Names

EAAT2, GLT1, SLC1A2

Entrez Gene IDs

6506 (Human)

Gene Symbol

SLC1A2

UniProt

NP_004162 (Human)

Additional EAAT2/GLT1 Products

Product Documents for EAAT2/GLT1 Antibody (1D8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EAAT2/GLT1 Antibody (1D8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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