EAAT2/GLT1 Antibody (28F9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26322

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26322-100ul
NBP3-26322-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 28F9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EAAT2/GLT1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human EAAT2/GLT1 [UniProt P43004]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EAAT2/GLT1 Antibody (28F9)

EAAT2/GLT1 Antibody (28F9)

Western Blot: EAAT2/GLT1 Antibody (28F9) [NBP3-26322] -

Western Blot: EAAT2/GLT1 Antibody (28F9) [NBP3-26322] - Positive Western Blot detected in: U251 whole cell lysate.
All lanes: EAAT2/GLT1 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 62 kDa
Observed band size: 62 kDa
EAAT2/GLT1 Antibody (28F9)

Flow Cytometry: EAAT2/GLT1 Antibody (28F9) [NBP3-26322] -

Flow Cytometry: EAAT2/GLT1 Antibody (28F9) [NBP3-26322] - Overlay Peak curve showing MCF-7 cells stained with EAAT2/GLT1 Antibody (28F9) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for EAAT2/GLT1 Antibody (28F9)

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: EAAT2/GLT1

GLT1 is the major glial glutamate transporter that takes up more than 90% of glutamate from the synapse. GLT1b is a splice variant that contains 11 unique amino acids at the C-terminus that encode a PDZ binding domain. GLT1b was initially believed to be the neuronal isoform of GLT1, but it now appears that GLT1b is localized to astrocytes in the normal brain and to neurons in pathological conditions. In Lou Gehrig's Disease, GLT1a is decreased, while GLT1b is increased. There are also different expressions in hypoxic cells, which have not yet been fully elucidated.

Long Name

Excitatory Amino Acid Transporter 2

Alternate Names

EAAT2, GLT1, SLC1A2

Gene Symbol

SLC1A2

Additional EAAT2/GLT1 Products

Product Documents for EAAT2/GLT1 Antibody (28F9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EAAT2/GLT1 Antibody (28F9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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