EAAT2/GLT1 Antibody - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20136

Novus Biologicals, part of Bio-Techne
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NBP1-20136
NBP1-20136SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human, Mouse, Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, In vivo assay, In-situ Hybridization, Western Blot

Cited:

Cytometric Bead Assay Standard, Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, In Situ Hybridization, In vivo assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all EAAT2/GLT1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide from mouse EAAT2/GLT1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.

Reactivity Notes

Marmoset

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EAAT2/GLT1 Antibody - Azide Free

Western Blot: EAAT2/GLT1 AntibodyAzide Free [NBP1-20136]

Western Blot: EAAT2/GLT1 AntibodyAzide Free [NBP1-20136]

Western Blot: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated overnight at 4C.
Immunocytochemistry/ Immunofluorescence: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136]

Immunocytochemistry/ Immunofluorescence: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136]

Immunocytochemistry/Immunofluorescence: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Human NPCs differentiate into mature neurons. Immunofluorescence staining of differentiated neurons derived from human dorsal NPCs (1323-2 line, day 35 after differentiation) for mature cortical neuronal markers expressed in the nucleus (BRN2, TBR1, NeuN) and cytoplasm (MAP2), glial markers (SOX9, GFAP, GLT1), and dorsal forebrain marker (FOXG1). Nuclei stained with DAPI, shown as an overlay over brightfield images. The merge is an overlay of the neuronal and glial markers. Scale bar 100 um. DAPI 4,6'-diamino-2-phenylindole Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-018-0812-6) licensed under a CC-BY license.
Immunohistochemistry: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136]

Immunohistochemistry: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136]

Immunohistochemistry: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Hippocampal GLT-1 and NR1 expression 3 days after SE with MyD88 inhibition. Sections from the hippocampi of mice in the CP group (A1-A3) and MIP group (B1-B3) 3 days after SE with GLT-1 immunoreactivity in astrocytes and neuronal processes. (A4, B4) Higher magnification of the boxes in (A3) and (B3). Scale bars: (A1-A3, B1-B3) 100 um; (A4, B4) 50 um. SE = status epilepticus, DG=dentate gyrus, hippocampal regions CA3 and CA1, CP = control peptide, MIP = MyD88 inhibitory peptide. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30112701/) licensed under a CC-BY license.
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Applications for EAAT2/GLT1 Antibody - Azide Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000
Application Notes
Although not tested this antibody may work in IHC-Frozen. Use in flow was reported in scientific literature (PMID: 23793269). Use in In-vivo and in ICC/IF reported in scientific literature (PMID 25581361). Use in In-situ Hybridization reported in scientific literature (PMID: 26150391).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Format

Azide Free

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EAAT2/GLT1

Mediates dihydrokainate-sensitive glutamate transport; may play a role in presynaptic terminal glutamate transport and synaptic transmission.; FUNCTION: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.; SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.; TISSUE SPECIFICITY: Localized in brain and is highly enriched in the Purkinje cell layer in cerebellum, retina.

Long Name

Excitatory Amino Acid Transporter 2

Alternate Names

EAAT2, GLT1, SLC1A2

Entrez Gene IDs

6506 (Human); 20511 (Mouse); 29482 (Rat)

Gene Symbol

SLC1A2

UniProt

P43006 (Mouse)

Additional EAAT2/GLT1 Products

Product Documents for EAAT2/GLT1 Antibody - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EAAT2/GLT1 Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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