EAAT2/GLT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84027

Novus Biologicals, part of Bio-Techne
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NBP1-84027-25ul
NBP1-84027
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EAAT2/GLT1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SLDAFLDLIRNLFPENLVQACFQQIQTVTKKVLVAPPPDEEANATSAVVSLLNETVTEVPEETKMVIKKGLEFKDGMNV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EAAT2/GLT1 Antibody

Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP1-84027]

Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP1-84027]

Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP1-84027] - Analysis in human cerebral cortex and fallopian tube tissues using NBP1-84027 antibody. Corresponding EAAT2/GLT1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP1-84027]

Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP1-84027]

Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP1-84027] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP1-84027]

Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP1-84027]

Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP1-84027] - Staining of human cerebral cortex shows high expression.
View 2 more images

Applications for EAAT2/GLT1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EAAT2/GLT1

GLT1 is the major glial glutamate transporter that takes up more than 90% of glutamate from the synapse. GLT1b is a splice variant that contains 11 unique amino acids at the C-terminus that encode a PDZ binding domain. GLT1b was initially believed to be the neuronal isoform of GLT1, but it now appears that GLT1b is localized to astrocytes in the normal brain and to neurons in pathological conditions. In Lou Gehrig's Disease, GLT1a is decreased, while GLT1b is increased. There are also different expressions in hypoxic cells, which have not yet been fully elucidated.

Long Name

Excitatory Amino Acid Transporter 2

Alternate Names

EAAT2, GLT1, SLC1A2

Gene Symbol

SLC1A2

Additional EAAT2/GLT1 Products

Product Documents for EAAT2/GLT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EAAT2/GLT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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