Key Product Details

Species Reactivity

Validated:

Rat

Predicted:

Bovine (100%), Mouse (100%), Rabbit (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4D6.2

Format

BSA Free

Concentration

1 mg/ml

View all EAAT3 Antibodies »

Product Summary for EAAT3 Antibody (4D6.2) - BSA Free

Immunogen

C-terminus peptide (KDKSDTISFTQTSQF).

Specificity

Glutamate Transporter EAAC1. Based on protein sequence the antibody is expected to react with mouse, bovine and rabbit.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Relevant Entrez Gene Numbers: NM_004170.4

Scientific Data Images for EAAT3 Antibody (4D6.2) - BSA Free

Immunohistochemistry: EAAT3 Antibody (4D6.2) - BSA Free [NBP2-29747]

Immunohistochemistry: EAAT3 Antibody (4D6.2) - BSA Free [NBP2-29747]

Immunohistochemistry: EAAT3 Antibody (4D6.2) - BSA Free [NBP2-29747] - Mouse anti-Glutamate Transporter [EAAC1] staining of rat cerebral cortex.

Applications for EAAT3 Antibody (4D6.2) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
EAAC1 is approximately 66-70kDa; the protein can form homomultimers during preparation as well, thus often times large 200-220kDa bands can also be seen.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

20mM Phosphate (pH 7.6) and 0.25M NaCl

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EAAT3

The EAAT3 gene encodes for glutamate transporters (also known as excitatory amino acid transporters, EAATs), that rapidly move glutamate from the postsynaptic cleft through the plasma membrane. The proteins are approximately 542 amino acids long at a mass barely over 57 kDA. This function allows for neurotoxic levels of glutamate in the brain to be avoided. It is common to see underactivity of glutamate receptors in some disorders such as ischemia and traumatic brain injuries and overactivity to be linked to mental illnesses such as schizophrenia. EAAT3 gene is also related to obsessive-compulsive disorder, ganglioglioma, autism spectrum disorders, aminoaciduriam temporal lobe epilepsy, hepatic encephalopathy, neuronitis, anorexia nervosa, and amyotrophic lateral sclerosis. It interacts with KNCN, HCCS, EWSR1, PRKCA, and ARL6IP5 to function in pathways of glutamic acid signaling, transmembrane transport of small molecules, and protein digestion and absorption.

Long Name

Excitatory amino acid transporter 3

Alternate Names

EAAC1, SLC1A1

Gene Symbol

SLC1A1

UniProt

P43005 (Human)

Additional EAAT3 Products

Product Documents for EAAT3 Antibody (4D6.2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EAAT3 Antibody (4D6.2) - BSA Free

Relevant Entrez Gene Numbers: NM_004170.4

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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