EBF-1 Antibody (1C12)
Novus Biologicals, part of Bio-Techne | Catalog # H00001879-M01J
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Sandwich ELISA Capture, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1C12
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
EBF1 (AAH38805, 1 a.a. ~ 591 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MFGIQESIQRSGSSMKEEPLGSGMNAVRTWMQGAGVLDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHFVLALYDRQGQPVEIERTAFVGFVEKEKEANSEKTNNGIHYRLQLLYSNGIRTEQDFYVRLIDSMTKQAIVYEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFSKFFLKCNQNCLKNAGNPRDMRRFQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEGTPSYLEHATPCIKAISPSEGWTTGGATVIIIGDNFFDGLQVIFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGRFIYTALNEPTIDYGFQRLQKVIPRHPGDPERLPKEVILKRAADLVEALYGMPHNNQEIILKRAADIAEALYSVPRNHNQLPALANTSVHAGMMGVNSFSGQLAVNVSEASQATNQGFTRNSSSVSPHGYVPSTTPQQTNYNSVTTSMNGYGSAAMSNLGGSPTFLNGSAANSPYAIVPSSPTMASSTSLPSNCSSSSGIFSFSPANMVSAVKQKSAFAPVVRPQTSPPPTCTSTNGNSLQAISGMIVPPM
Specificity
EBF1 - early B-cell factor 1 (1C12)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for EBF-1 Antibody (1C12)
![Western Blot: EBF-1 Antibody (1C12) [H00001879-M01J]](https://resources.bio-techne.com/images/products/EBF-1-Antibody-1C12-Western-Blot-H00001879-M01J-img0002.jpg "Western Blot: EBF-1 Antibody (1C12) [H00001879-M01J]")
Western Blot: EBF-1 Antibody (1C12) [H00001879-M01J]
Western Blot: EBF-1 Antibody (1C12) [H00001879-M01J] - Analysis of EBF1 expression in transfected 293T cell line by EBF1 monoclonal antibody (M01A), clone 1C12.Lane 1: EBF1 transfected lysate(64 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: EBF-1 Antibody (1C12) [H00001879-M01J] - Detection limit for recombinant GST tagged EBF1 is 0.3 ng/ml as a capture antibody.
![Sandwich ELISA Capture: EBF-1 Antibody (1C12) [H00001879-M01J]](https://resources.bio-techne.com/images/products/EBF-1-Antibody-1C12-Sandwich-ELISA-Capture-H00001879-M01J-img0003.jpg "Sandwich ELISA Capture: EBF-1 Antibody (1C12) [H00001879-M01J]")
Sandwich ELISA Capture: EBF-1 Antibody (1C12) [H00001879-M01J]
Sandwich ELISA Capture: EBF-1 Antibody (1C12) [H00001879-M01J] - Detection limit for recombinant GST tagged EBF-1 is 0.1 ng/ml as a capture antibody.Applications for EBF-1 Antibody (1C12)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: EBF-1
Long Name
Early B-cell Factor 1
Alternate Names
COE1, EBF1, OE-1, OLF1
Entrez Gene IDs
1879 (Human)
Gene Symbol
EBF1
UniProt
Additional EBF-1 Products
Product Documents for EBF-1 Antibody (1C12)
Product Specific Notices for EBF-1 Antibody (1C12)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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