Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Tissue Culture Substratum, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 15k8D10

Format

BSA Free

Concentration

1.0 mg/ml

View all EBI3 Antibodies »

Product Specifications

Immunogen

A full-length human Interleukin-35-Fc fusion protein was used as immunogen for this antibody.

Specificity

The human Interleukin-35 protein is a dimeric protein consisting of the human IL-12a/p35 subunit (NP_000873.2) and the human EBI3 (NP_005746) protein. Clone 15K8D10 tests as being specific to the EBI3 subunit of the target protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Endotoxin Level

< 0.06 EU/ug

Scientific Data Images for EBI3 Antibody (15k8D10) - BSA Free

Western Blot: EBI3 Antibody (15k8D10)BSA Free [NBP2-27362]

Western Blot: EBI3 Antibody (15k8D10)BSA Free [NBP2-27362]

Western Blot: EBI3 Antibody (15k8D10) [NBP2-27362] - Analysis of IL-35-Fc protein (A) and human Fc (B) using IL-35 antibody at 4ug/ml.
Immunohistochemistry-Paraffin: EBI3 Antibody (15k8D10) - BSA Free [NBP2-27362]

Immunohistochemistry-Paraffin: EBI3 Antibody (15k8D10) - BSA Free [NBP2-27362]

Immunohistochemistry-Paraffin: EBI3 Antibody (15k8D10) [NBP2-27362] - Formalin-fixed, paraffin-embedded human spleen (left) and lymph node metastatic malignant melanoma (right) stained with IL-35 antibody (5 ug/ml) using peroxidase-conjugate and DAB chromogen.

Applications for EBI3 Antibody (15k8D10) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 31601798)

Immunohistochemistry

reported in scientific literature (PMID 27681506)

Immunohistochemistry-Paraffin

5ug/ml

Western Blot

3-5ug/ml~

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.1 ml PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EBI3

IL-35 is a novel heterodimeric cytokine composed of the IL-12p35 subunit and the Epstein-Barr virus-induced gene 3 (EBI3) protein. As one of the IL-12 family members, IL-35 is known to play an essential role in immune regulation of the CD4+CD25+ regulatory T (Treg) cells, from which IL-35 is also specifically produced. IL-35 is able to expand CD4+CD25+ Treg cells whereas it can inhibit proliferation of CD4+CD25- effector T (Teff) cells and development of proinflammatory Th17 cells, suppressing inflammatory responses.

Long Name

Epstein-Barr virus induced gene 3

Alternate Names

EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3

Gene Symbol

EBI3

UniProt

NP_000873.2 (Human)

Additional EBI3 Products

Product Documents for EBI3 Antibody (15k8D10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EBI3 Antibody (15k8D10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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