Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all EBI3 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of mature human EBI-3. The immunogen is located within the last 50 amino acids of EBI3. Amino Acid Squence: ILRAVRPRATYYIQV

Specificity

Human EBI3 has only 1 isoform (229aa, 25kD). Mouse EBI3 has one isoform (228aa, 25kD) and rat EBI3 also has one isoform (228aa, 25kD). NBP1-76976 can detect human EBI3 but cannot detect mouse and rat EBI3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EBI3 Antibody - BSA Free

Western Blot: EBI3 AntibodyBSA Free [NBP1-76976]

Western Blot: EBI3 AntibodyBSA Free [NBP1-76976]

Western Blot: EBI3 Antibody [NBP1-76976] - Analysis of EBI3 in human tonsil tissue lysate with EBI3 antibody at 2 ug/mL.
Western Blot: EBI3 AntibodyBSA Free [NBP1-76976]

Western Blot: EBI3 AntibodyBSA Free [NBP1-76976]

Western Blot: EBI3 Antibody [NBP1-76976] - K562 lysate with EBI3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
EBI3 Antibody - BSA Free

Western Blot: EBI3 Antibody - BSA Free [NBP1-76976] -

Western Blot: EBI3 Antibody - BSA Free [NBP1-76976] - Recombinant Protein Validation. Loading: 30 ng of human recombinant IL-17 (IL-17A+IL-17B) protein per lane.Antibodies: EBI-3 , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 ug/mL, Lane 2: 2 ug/mL, Lane 3: 4 ug/mL,
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Applications for EBI3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1-4 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EBI3

EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35 (1-3). Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naive but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.

Long Name

Epstein-Barr virus induced gene 3

Alternate Names

EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3

Gene Symbol

EBI3

UniProt

NP_005746

Additional EBI3 Products

Product Documents for EBI3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EBI3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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