EEA1 Antibody (GT10811)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13599

Novus Biologicals, part of Bio-Techne
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NBP3-13599
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT10811

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EEA1 Antibodies »

Product Summary for EEA1 Antibody (GT10811)

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EEA1. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

162 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for EEA1 Antibody (GT10811)

Western Blot: EEA1 Antibody (GT10811) [NBP3-13599]

Western Blot: EEA1 Antibody (GT10811) [NBP3-13599]

Western Blot: EEA1 Antibody (GT10811) [NBP3-13599] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EEA1 antibody [GT10811] (NBP3-13599) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: EEA1 Antibody (GT10811) [NBP3-13599]

Immunocytochemistry/ Immunofluorescence: EEA1 Antibody (GT10811) [NBP3-13599]

Immunocytochemistry/Immunofluorescence: EEA1 Antibody (GT10811) [NBP3-13599] - EEA1 antibody [GT10811] detects EEA1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EEA1 stained by EEA1 antibody [GT10811] (NBP3-13599) diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: EEA1 Antibody (GT10811) [NBP3-13599]

Immunohistochemistry-Paraffin: EEA1 Antibody (GT10811) [NBP3-13599]

Immunohistochemistry-Paraffin: EEA1 Antibody (GT10811) [NBP3-13599] - EEA1 antibody [GT10811] detects EEA1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. EEA1 stained by EEA1 antibody [GT10811] (NBP3-13599) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications for EEA1 Antibody (GT10811)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EEA1

EEA1 binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking

Long Name

Early Endosome Antigen 1

Alternate Names

MST105, MSTP105, ZFYVE2

Gene Symbol

EEA1

Additional EEA1 Products

Product Documents for EEA1 Antibody (GT10811)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EEA1 Antibody (GT10811)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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