EEA1 Antibody (M347)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23301
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M347
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Clone M347 was generated from a recombinant protein corresponding to amino acid residues in the N-terminal region of human EEA1. This sequence has high homology to similar regions in rat and mouse EEA1. [UniProt# Q15075]
Specificity
This antibody detects a 180 kDa protein corresponding to the apparent molecular mass of EEA1 on SDS-PAGE immunoblots of adult mouse brain. In immunocytochemistry, anti-EEA1 specifically stains endosomal vesicles.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for EEA1 Antibody (M347)
Western Blot: EEA1 Antibody (M347) [NBP3-23301] -
Western Blot: EEA1 Antibody (M347) [NBP3-23301] - Western blot of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-EEA1 antibody at 1:500 (lane 1) or 1:1000 (lane 2).
Immunocytochemistry/Immunofluorescence: EEA1 Antibody (M347) [NBP3-23301] -
Immunocytochemistry/Immunofluorescence: EEA1 Antibody (M347) [NBP3-23301] - Immunocytochemical labeling of EEA1 in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The cells were labeled with mouse monoclonal EEA1 (NBP3-23301). The antibody was detected using goat anti-mouse DyLight(R) 488.Applications for EEA1 Antibody (M347)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: EEA1
Long Name
Early Endosome Antigen 1
Alternate Names
MST105, MSTP105, ZFYVE2
Gene Symbol
EEA1
Additional EEA1 Products
Product Documents for EEA1 Antibody (M347)
Product Specific Notices for EEA1 Antibody (M347)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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