EED Antibody (GT885)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13628

Novus Biologicals, part of Bio-Techne
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NBP3-13628
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chicken (99%), Fish (95%), Rhesus Macaque (100%), Xenopus (96%), Zebrafish (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT885

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EED Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human EED. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for EED Antibody (GT885)

Western Blot: EED Antibody (GT885) [NBP3-13628]

Western Blot: EED Antibody (GT885) [NBP3-13628]

Western Blot: EED Antibody (GT885) [NBP3-13628] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EED antibody [GT885] (NBP3-13628) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: EED Antibody (GT885) [NBP3-13628]

Immunocytochemistry/ Immunofluorescence: EED Antibody (GT885) [NBP3-13628]

Immunocytochemistry/Immunofluorescence: EED Antibody (GT885) [NBP3-13628] - EED antibody [GT885] detects EED protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EED stained by EED antibody [GT885] (NBP3-13628) diluted at 1:1000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: EED Antibody (GT885) [NBP3-13628]

Immunohistochemistry-Paraffin: EED Antibody (GT885) [NBP3-13628]

Immunohistochemistry-Paraffin: EED Antibody (GT885) [NBP3-13628] - EED antibody [GT885] detects EED protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat endometrial carcinoma. EED stained by EED antibody [GT885] (NBP3-13628) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications for EED Antibody (GT885)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EED

EED encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Embryonic Ectoderm Development

Alternate Names

WAIT1

Gene Symbol

EED

Additional EED Products

Product Documents for EED Antibody (GT885)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EED Antibody (GT885)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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