EEF1B2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00341

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EEF1B2 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human EEF1B2 (Accession#: P24534; Met1-Ile225)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for EEF1B2 Antibody

Western Blot: EEF1B2 Antibody [NBP3-00341]

Western Blot: EEF1B2 Antibody [NBP3-00341]

Western Blot: EEF1B2 Antibody [NBP3-00341] - Anti-EEF1B2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 32 kDa (We are unsure as to the identity of these extra bands).
Immunohistochemistry-Paraffin: EEF1B2 Antibody [NBP3-00341]

Immunohistochemistry-Paraffin: EEF1B2 Antibody [NBP3-00341]

Immunohistochemistry-Paraffin: EEF1B2 Antibody [NBP3-00341] - Immunochemical staining of human EEF1B2 in human breast carcinoma with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).
Immunoprecipitation: EEF1B2 Antibody [NBP3-00341]

Immunoprecipitation: EEF1B2 Antibody [NBP3-00341]

Immunoprecipitation: EEF1B2 Antibody [NBP3-00341] - EEF1B2 was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. Lane B: 0.5 mg K562 Whole Cell Lysate. 2 ul anti-EEF1B2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-EEF1B2 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDa

Applications for EEF1B2 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:2500-1:10000

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EEF1B2

EEF1B2 encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR.

Alternate Names

EEF1B, EEF1B1, EF1B, EF-1-beta, elongation factor 1-beta, eukaryotic translation elongation factor 1 beta 1, eukaryotic translation elongation factor 1 beta 2

Gene Symbol

EEF1B2

Additional EEF1B2 Products

Product Documents for EEF1B2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EEF1B2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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