EEN Antibody (2B5)
Novus Biologicals, part of Bio-Techne | Catalog # H00006455-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2B5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SH3GL1 (NP_003016, 12 a.a. ~ 119 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KASQLVSEKVGGAEGTKLDDDFKEMEKKVDVTSKAVTEVLARTIEYLQPNPASRAKLTMLNTVSKIRGQVKNPGYPQSEGLLGECMIRHGKELGGESNFGDALLDAGE
Specificity
SH3GL1 - SH3-domain GRB2-like 1 (2B5)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for EEN Antibody (2B5)
![Western Blot: EEN Antibody (2B5) [H00006455-M02]](https://resources.bio-techne.com/images/products/EEN-Antibody-2B5-Western-Blot-H00006455-M02-img0002.jpg "Western Blot: EEN Antibody (2B5) [H00006455-M02]")
Western Blot: EEN Antibody (2B5) [H00006455-M02]
Western Blot: EEN Antibody (2B5) [H00006455-M02] - SH3GL1 monoclonal antibody (M02), clone 2B5. Analysis of SH3GL1 expression in HeLa.
Sandwich ELISA: EEN Antibody (2B5) [H00006455-M02] - Detection limit for recombinant GST tagged SH3GL1 is 3 ng/ml as a capture antibody.
Applications for EEN Antibody (2B5)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: EEN
Alternate Names
CNSA1endophilin-2, EEN fusion partner of MLL, EENSH3 domain-containing GRB2-like protein 1, Endophilin-2, extra 11-19 leukemia fusion, Extra eleven-nineteen leukemia fusion gene protein, MGC111371, SH3 domain protein 2B, SH3-containing Grb-2-like 1 protein, SH3D2Bendophilin-A2, SH3-domain GRB2-like 1, SH3P8
Entrez Gene IDs
6455 (Human)
Gene Symbol
SH3GL1
UniProt
Additional EEN Products
Product Documents for EEN Antibody (2B5)
Product Specific Notices for EEN Antibody (2B5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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