Skip to main content

EFHD2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1477

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NB100-1477
NB100-1477-25ug

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence CAAFKELQSTFK corresponding to C-Terminus according to NP_077305.2.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23959172).

Specificity

This antibody does not cross-react with Mouse Swiprosin 2. Data kindly provided by Sebastian Duetting and Dirk Mielenz.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EFHD2 Antibody

Western Blot: EFHD2 Antibody [NB100-1477]

Western Blot: EFHD2 Antibody [NB100-1477]

Western Blot: EFHD2 Antibody [NB100-1477] - Staining of Human Thymus, Human Lymph node and Human Tonsil lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: EFHD2 Antibody [NB100-1477]

Immunohistochemistry-Paraffin: EFHD2 Antibody [NB100-1477]

Immunohistochemistry-Paraffin: EFHD2 Antibody [NB100-1477] - Analysis of Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for EFHD2 Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 4 ug/ml

Immunohistochemistry-Paraffin

2 - 4 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 10 ug/ml
Application Notes
WB: Approx. 30 kDa band observed in human tonsil, thymus and lymph node and in mouse thymus and lymph node lysates (calculated MW of 26.7 kDa band according to NP_077305.2). IHC-P: Human tonsil shows membranous staining in selected cells.

Reviewed Applications

Read 1 review rated 5 using NB100-1477 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EFHD2

Found in lymphocytes; preferentially expressed in CD8+ cells. Contains 2 EF Hand domains.

Alternate Names

EF hand domain containing 2, EF hand domain family, member D2, EF-hand domain family, member D2, EF-hand domain-containing protein D2, MGC4342, swiprosin 1, swiprosin-1, SWS1

Entrez Gene IDs

79180 (Human); 27984 (Mouse)

Gene Symbol

EFHD2

UniProt

Additional EFHD2 Products

Product Documents for EFHD2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EFHD2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...