EGLN3/PHD3 Antibody (EG188e/d5) [DyLight 680]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30440FR

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-30440AF350

Alexa Fluor 405

NBP1-30440AF405

Alexa Fluor 488

NBP1-30440AF488

Alexa Fluor 532

NBP1-30440AF532

Alexa Fluor 594

NBP1-30440AF594

Alexa Fluor 647

NBP1-30440AF647

Alexa Fluor 700

NBP1-30440AF700

Alexa Fluor 750

NBP1-30440AF750

Allophycocyanin

NBP1-30440APC

Biotin

NBP1-30440B

DyLight 350

NBP1-30440UV

DyLight 405

NBP1-30440V

DyLight 488

NBP1-30440G

DyLight 550

NBP1-30440R

DyLight 594

NBP1-30440DL594

DyLight 650

NBP1-30440C

DyLight 755

NBP1-30440IR

FITC

NBP1-30440F

HRP

NBP1-30440H

Janelia Fluor 525

NBP1-30440JF525

Janelia Fluor 549

NBP1-30440JF549

Janelia Fluor 635

NBP1-30440JF635

Janelia Fluor 646

NBP1-30440JF646

Janelia Fluor 669

NBP1-30440JF669

PE

NBP1-30440PE

PerCP

NBP1-30440PCP

Forumulation

Catalog #

BSA Free

NBP1-30440

Azide and BSA Free

NBP2-80715

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

DyLight 680 (Excitation = 692 nm, Emission = 712 nm)

Antibody Source

Monoclonal Mouse IgG₁ Clone # EG188e/d5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EGLN3/PHD3 Antibodies »

Product Specifications

Immunogen

Partial recombinant human PHD3/HIF Prolyl Hydroxylase 3 (UniProt# Q9H6Z9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for EGLN3/PHD3 Antibody (EG188e/d5) [DyLight 680]

Application

Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: EGLN3/PHD3

HIF prolyl hydroxylase 3 (HPH-3; PHD3; EGLN3) catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, hydroxylates HIF-1 alpha at Pro-564, and also hydroxylates HIF-2 alpha. PHD3 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. EGLN3 may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. HIF prolyl hydroxylase 3 promotes cell death through a caspase-dependent mechanism.

Long Name

Egl Nine Homolog 3/Prolyl Hydroxylase Domain-containing Protein 3

Alternate Names

HIFPH3, PHD3

Gene Symbol

EGLN3

Additional EGLN3/PHD3 Products

Product Documents for EGLN3/PHD3 Antibody (EG188e/d5) [DyLight 680]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for EGLN3/PHD3 Antibody (EG188e/d5) [DyLight 680]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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