EI2BL Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06310
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human EI2BL fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for EI2BL Antibody
Western Blot: EI2BL Antibody [NBP3-06310]
Western Blot: EI2BL Antibody [NBP3-06310] - EI2BL antibody rabbit polyclonal antibody at 1:500 dilutionLane A: RT-4 Whole Cell LysateLane B: U-251MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:39 kDa(We are unsure as to the identity of these extra bands.)Immunocytochemistry/ Immunofluorescence: EI2BL Antibody [NBP3-06310]
Immunocytochemistry/Immunofluorescence: EI2BL Antibody [NBP3-06310] - Immunofluorescence staining of EI2BL antibody in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MRI1 polyclonal antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.Immunohistochemistry-Paraffin: EI2BL Antibody [NBP3-06310]
Immunohistochemistry-Paraffin: EI2BL Antibody [NBP3-06310] - Immunochemical staining of human EI2BL antibody in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.Applications for EI2BL Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:50-1:200
Immunoprecipitation
1-5uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EI2BL
Alternate Names
EC 5.3.1.23, M1Pi, Mediator of RhoA-dependent invasion, methylthioribose-1-phosphate isomerase, methylthioribose-1-phosphate isomerase homolog (S. cerevisiae), MGC3207, MRDI, MTNA, MTR-1-P isomerase, S-methyl-5-thioribose-1-phosphate isomerase, Translation initiation factor eIF-2B subunit alpha/beta/delta-like protein, Ypr118w
Gene Symbol
MRI1
Additional EI2BL Products
Product Specific Notices for EI2BL Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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