eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6)

Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] -

Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] -

Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] -

Western Blot: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Positive Western Blot detected in Hela whole cell lysate (treated with Calyculin A or EGF).
All lanes: eIF2 alpha/EIF2S1 [p Ser51] Antibody at 1.48ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 36 KDa
Observed band size: 36 KDa