eIF2 alpha/EIF2S1 Antibody (PSH04-29)

Western Blot: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296]

Western Blot: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Western blot analysis of eIF2 alpha/EIF2S1 on different lysates with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/2,000 dilution.

Lane 1: MCF7 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HeLa cell lysate
Lane 4: COS-1 cell lysate
Lane 5: A549 cell lysate
Lane 6: RAW264.7 cell lysate
Lane 7: C6 cell lysate
Lane 8: Mouse kidney tissue lysate
Lane 9: Mouse spleen tissue lysate
Lane 10: Rat kidney tissue lysate
Lane 11: Rat spleen tissue lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 59 seconds; ECL;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32296) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296]

Immunohistochemistry: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32296) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296]

Immunocytochemistry/ Immunofluorescence: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Immunocytochemistry analysis of HepG2 cells labeling eIF2 alpha/EIF2S1 with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.