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eIF2 alpha/EIF2S1 [p Ser51] Antibody (SR1604)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21506

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-21506-100ul
NBP3-21506-50ul

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1604

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-eIF2 alpha/EIF2S1 [Ser51] (Uniprot #: P05198)

Modification

p Ser51

Specificity

Detects endogenous levels of total phospho-eIF2 alpha/EIF2S1 [Ser51]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF2 alpha/EIF2S1 [p Ser51] Antibody (SR1604)

Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser 51] Antibody (SR1604) [NBP3-21506] -

Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser 51] Antibody (SR1604) [NBP3-21506] - Immunohistochemical analysis of paraffin-embedded human colon cancer, using NBP3-21506.

Western Blot: eIF2 alpha/EIF2S1 [p Ser 51] Antibody (SR1604) [NBP3-21506] -

Western Blot: eIF2 alpha/EIF2S1 [p Ser 51] Antibody (SR1604) [NBP3-21506] - Western blot analysis using NBP3-21506 in (1)HeLa cell lysates treated with Calyculin A;(2) Untreated HeLa cell lysates.

Applications for eIF2 alpha/EIF2S1 [p Ser51] Antibody (SR1604)

Application
Recommended Usage

Flow Cytometry

1:40

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF2 alpha

eIFs are heterotrimeric initiation complexes that form on the ribosome during protein synthesis. Each eIF serves a distinct function during protein synthesis to help attach, assemble, and drive the ribosome along the mRNA.

Long Name

Eukaryotic Translation Initiation Factor 2 Alpha

Alternate Names

EIF2S1

Gene Symbol

EIF2S1

Additional eIF2 alpha Products

Product Documents for eIF2 alpha/EIF2S1 [p Ser51] Antibody (SR1604)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for eIF2 alpha/EIF2S1 [p Ser51] Antibody (SR1604)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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