eIF2 alpha/EIF2S1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2249

Novus Biologicals, part of Bio-Techne
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NB100-2249
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Primate (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all eIF2 alpha Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 275 and the C-terminus (residue 315) of human Eukarytic Translation Initiation Factor 1, subunit 1 alpha, 35kDa using the numbering given in entry NP_004085.1 (GeneID 1965).

Reactivity Notes

Orangutan (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF2 alpha/EIF2S1 Antibody

Western Blot: eIF2 alpha/EIF2S1 Antibody [NB100-2249]

Western Blot: eIF2 alpha/EIF2S1 Antibody [NB100-2249]

Western Blot: eIF2 alpha/EIF2S1 Antibody [NB100-2249] - Detection of Human and Mouse eIF2-alpha on HeLa whole cell lysate using NB100-2249.
Immunohistochemistry-Paraffin: eIF2 alpha/EIF2S1 Antibody [NB100-2249]

Immunohistochemistry-Paraffin: eIF2 alpha/EIF2S1 Antibody [NB100-2249]

Immunohistochemistry-Paraffin: eIF2 alpha/EIF2S1 Antibody [NB100-2249] - Human colon carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-eIF2alpha/EIF2S1 used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB

Applications for eIF2 alpha/EIF2S1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: eIF2 alpha

EIF2S1, also known as eIF2a, is the alpha subunit of the translation initiation factor eIF2 complex which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. The phosphorylation state of eIF2S1 controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2S1 is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation. Recombinant human EIF2S1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Eukaryotic Translation Initiation Factor 2 Alpha

Alternate Names

EIF2S1

Entrez Gene IDs

1965 (Human); 13665 (Mouse)

Gene Symbol

EIF2S1

UniProt

P05198

Additional eIF2 alpha Products

Product Documents for eIF2 alpha/EIF2S1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for eIF2 alpha/EIF2S1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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