eIF2 alpha/EIF2S1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32289

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (98%), Rhesus Macaque (100%), Xenopus (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all eIF2 alpha Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human eIF2 alpha/EIF2S1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (92%), Chicken (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF2 alpha/EIF2S1 Antibody

Western Blot: eIF2 alpha/EIF2S1 Antibody [NBP1-32289]

Western Blot: eIF2 alpha/EIF2S1 Antibody [NBP1-32289]

Western Blot: eIF2 alpha/EIF2S1 Antibody [NBP1-32289] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with eIF2 alpha antibody [N1C3] diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: eIF2 alpha/EIF2S1 Antibody [NBP1-32289]

Immunocytochemistry/ Immunofluorescence: eIF2 alpha/EIF2S1 Antibody [NBP1-32289]

Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 Antibody [NBP1-32289] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF2 alpha protein stained by eIF2 alpha antibody [N1C3] diluted at 1:5000. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunocytochemistry/ Immunofluorescence: eIF2 alpha/EIF2S1 Antibody [NBP1-32289]

Immunocytochemistry/ Immunofluorescence: eIF2 alpha/EIF2S1 Antibody [NBP1-32289]

Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 Antibody [NBP1-32289] - HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF2 alpha protein stained by eIF2 alpha antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.

Applications for eIF2 alpha/EIF2S1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: eIF2 alpha

EIF2S1, also known as eIF2a, is the alpha subunit of the translation initiation factor eIF2 complex which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. The phosphorylation state of eIF2S1 controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2S1 is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation. Recombinant human EIF2S1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Eukaryotic Translation Initiation Factor 2 Alpha

Alternate Names

EIF2S1

Gene Symbol

EIF2S1

Additional eIF2 alpha Products

Product Documents for eIF2 alpha/EIF2S1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for eIF2 alpha/EIF2S1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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