eIF2 alpha/EIF2S1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49428

Novus Biologicals, part of Bio-Techne
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NBP2-49428
NBP2-49428-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all eIF2 alpha Antibodies »

Product Summary for eIF2 alpha/EIF2S1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: IAPPRYVMTTTTLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLERENAEVDG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF2 alpha/EIF2S1 Antibody

Western Blot: eIF2 alpha/EIF2S1 Antibody [NBP2-49428]

Western Blot: eIF2 alpha/EIF2S1 Antibody [NBP2-49428]

Western Blot: eIF2 alpha/EIF2S1 Antibody [NBP2-49428] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Lane 4: Human plasma
Lane 5: Human Liver tissue
Lane 6: Human Tonsil tissue
Immunocytochemistry/ Immunofluorescence: eIF2 alpha/EIF2S1 Antibody [NBP2-49428]

Immunocytochemistry/ Immunofluorescence: eIF2 alpha/EIF2S1 Antibody [NBP2-49428]

Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 Antibody [NBP2-49428] - Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
Immunohistochemistry-Paraffin: eIF2 alpha/EIF2S1 Antibody [NBP2-49428]

Immunohistochemistry-Paraffin: eIF2 alpha/EIF2S1 Antibody [NBP2-49428]

Immunohistochemistry-Paraffin: eIF2 alpha/EIF2S1 Antibody [NBP2-49428] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Applications for eIF2 alpha/EIF2S1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF2 alpha

eIFs are heterotrimeric initiation complexes that form on the ribosome during protein synthesis. Each eIF serves a distinct function during protein synthesis to help attach, assemble, and drive the ribosome along the mRNA.

Long Name

Eukaryotic Translation Initiation Factor 2 Alpha

Alternate Names

EIF2S1

Gene Symbol

EIF2S1

Additional eIF2 alpha Products

Product Documents for eIF2 alpha/EIF2S1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eIF2 alpha/EIF2S1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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