eIF2A Antibody (3A7B11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80716

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3A7B11

Format

Azide and BSA Free

Concentration

1 mg/ml

View all eIF2A Antibodies »

Product Specifications

Immunogen

Partial recombinant human EIF2A (between residues 350-585) expressed in E. coli [Uniprot Q9BY44]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for eIF2A Antibody (3A7B11) - Azide and BSA Free

Western Blot: eIF2A Antibody (3A7B11)Azide and BSA Free [NBP2-80716]

Western Blot: eIF2A Antibody (3A7B11)Azide and BSA Free [NBP2-80716]

Western Blot: eIF2A Antibody (3A7B11) - Azide and BSA Free [NBP2-80716] - Western blot analysis of HeLa (1), NIH3T3 (2), A431 (3), MCF-7 (4), Ntera2 (5), PC12 (6), HepG2 (7), and Cos7 (8) cell lysate using eIF2A antibody at a concentration of 2 ug/ml. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: eIF2A Antibody (3A7B11) - Azide and BSA Free [NBP2-80716]

Immunocytochemistry/ Immunofluorescence: eIF2A Antibody (3A7B11) - Azide and BSA Free [NBP2-80716]

Immunocytochemistry/Immunofluorescence: eIF2A Antibody (3A7B11) - Azide and BSA Free [NBP2-80716] - eIF2A antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. Image from the standard format of this antibody.
Immunohistochemistry: eIF2A Antibody (3A7B11) - Azide and BSA Free [NBP2-80716]

Immunohistochemistry: eIF2A Antibody (3A7B11) - Azide and BSA Free [NBP2-80716]

Immunohistochemistry: eIF2A Antibody (3A7B11) - Azide and BSA Free [NBP2-80716] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human breast cancer using eIF2A (3A7B11) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrat

Applications for eIF2A Antibody (3A7B11) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF2A

eIF-2 alpha (also known as eukaryotic translation initiation factor 2, subunit 1 alpha) is a 35 kD member of the eukaryotic translation initiation factor 2 family. This cytoplasmic protein is ubiquitously expressed and can downregulate protein synthesis when phosphorylated by PKR kinase under stress conditions (hypoxia, viral infection, ER stress). Increased expression of eIF-2 alpha has been linked to progression of thyroid carcinoma.Signaling inhibited by p58 (IPK) and PKR phosphorylation. Can attenuate the unfolded protein response and inhibits translation. This protein has been shown to interact with eIF2B alpha, eIF2B delta, eIF2B beta subunit 2, p67, p68, and PKR. eIF-2 alpha is a target for cleavage by caspase 3, caspase 6, caspase 8, and caspase 10.

Alternate Names

EIF-2, EIF-2A, EIF2AEIF2, eIF-2alpha, eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa, subunit 1 (alpha, 35kD )

Gene Symbol

EIF2A

Additional eIF2A Products

Product Documents for eIF2A Antibody (3A7B11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for eIF2A Antibody (3A7B11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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