eIF2A [p Ser51] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81896

Novus Biologicals, part of Bio-Techne
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NB100-81896
NB100-81896-0.025mg
NB100-81896-0.1mg
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Canine, Fish

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all eIF2A Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human eIF2A around the phosphorylation site of serine 51 (E-L-SP-R-R).

Modification

p Ser51

Specificity

Detects endogenous levels of eIF2A only when phosphorylated at serine 51.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF2A [p Ser51] Antibody

Western Blot: eIF2A [p Ser51] Antibody [NB100-81896]

Western Blot: eIF2A [p Ser51] Antibody [NB100-81896]

Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2 alpha (Phospho-Ser51) antibody.

Immunocytochemistry/Immunofluorescence: eIF2A [p Ser51] Antibody [NB100-81896] -

Immunocytochemistry/Immunofluorescence: eIF2A [p Ser51] Antibody [NB100-81896] - Staining of methanol-fixed HeLa cells using NB100-81896
Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896]

Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896]

Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] - Paraffin-embedded human lung carcinoma tissue.
View 2 more images

Applications for eIF2A [p Ser51] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-200

Immunohistochemistry

1:100-1:300

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% glycerol, 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF2A

eIF2 alpha is a 36 kDa protein which is ubiquitously expressed in many cell types. The eIF2 protein, which is composed of three subunits (alpha, beta and gamma), is one of the key molecules in the initiation of translation. In mammalian cells, eIF2 alpha is phosphorylated at serine 51 (human EIF2 alpha, the equivalent residue in mouse is serine 52) by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF2 alpha blocks the GDP-GTP exchange activity of eIF2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF2 alpha is an important regulatory process in protein synthesis.

Alternate Names

EIF-2, EIF-2A, EIF2AEIF2, eIF-2alpha, eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa, subunit 1 (alpha, 35kD )

Entrez Gene IDs

83939 (Human); 229317 (Mouse); 502531 (Rat)

Gene Symbol

EIF2A

UniProt

P05198

Additional eIF2A Products

Product Documents for eIF2A [p Ser51] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eIF2A [p Ser51] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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